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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Comparative performances of HIV-1 RNA load assays at low viral load levels: Results of an international collaboration
Journal of Clinical Microbiology, Volume 52, No. 2, Year 2014
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Description
Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of ∼ 100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Swenson, Luke C.
Canada, Vancouver
British Columbia Centre for Excellence in Hiv-aids
Cobb, Bryan
United States, Pleasanton
Roche Molecular Systems
Geretti, Anna María
United Kingdom, Liverpool
University of Liverpool
Harrigan, P. Richard
Canada, Vancouver
British Columbia Centre for Excellence in Hiv-aids
Poljak, Mario
Slovenia, Ljubljana
Univerza V Ljubljani
Seguin-Devaux, Carole
Luxembourg, Strassen
Luxembourg Institute of Health
Verhofstede, Chris
Belgium, Ghent
Universiteit Gent
Wirden, Marc
France, Paris
Ap-hp Assistance Publique - Hopitaux de Paris
Amendola, Alessandra
Italy, Rome
Irccs Istituto Nazionale Malattie Infettive Lazzaro Spallanzani
Böni, Jürg
Switzerland, Zurich
Universität Zürich
Bourlet, Thomas
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Huder, Jon B.
Switzerland, Zurich
Universität Zürich
Karasi, Jean Claude
Rwanda, Kigali
National Reference Laboratory
Zidovec Lepej, Snjezana
Croatia, Zagreb
University Hospital for Infectious Diseases dr Fran Mihaljevic
Lunar, Maja M.
Slovenia, Ljubljana
Univerza V Ljubljani
Mukabayire, Odette
Rwanda, Kigali
National Reference Laboratory
Schuurman, Rob J.
Netherlands, Utrecht
University Medical Center Utrecht
Tomažič, Janez
Slovenia, Ljubljana
Univerza V Ljubljani
van Laethem, Kristel V.
Belgium, Leuven
Rega Institute for Medical Research
Vandekerckhove, Linos P.R.
Belgium, Ghent
Universiteit Gent
Wensing, Annemarie Marie J.
Netherlands, Utrecht
University Medical Center Utrecht
Statistics
Citations: 54
Authors: 21
Affiliations: 14
Identifiers
Doi:
10.1128/JCM.02461-13
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Infectious Diseases