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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum
Plant Cell, Volume 19, No. 11, Year 2007
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Description
Stagonospora nodorum is a major necrotrophic fungal pathogen of wheat (Triticum aestivum) and a member of the Dothideomycetes, a large fungal taxon that includes many important plant pathogens affecting all major crop plant families. Here, we report the acquisition and initial analysis of a draft genome sequencefor this fungus. The assembly comprises 37,164,227 bp of nuclear DNA contained in 107 scaffolds. The circular mitochondrial genome comprises 49,761 bp encoding 46 genes, including four that are intron encoded. The nuclear genome assembly contains 26 classes of repetitive DNA, comprising 4.5%of the genome. Some of the repeats show evidence of repeat-induced point mutations consistent with a frequent sexual cycle. ESTs and gene prediction models support aminimum of 10,762 nuclear genes. Extensive orthology was found between the polyketide synthase family in S. nodorum and Cochliobolus heterostrophus, suggesting an ancient origin and conserved functions for these genes. A striking feature of the gene catalogwas the large number of genes predicted to encode secreted proteins; the majority has no meaningful similarity to any other known genes. It is likely that genes for host-specific toxins, in addition to ToxA, will be found among this group. ESTs obtained from axenic mycelium grown on oleate (chosen tomimic early infection) and late-stage lesions sporulating on wheat leaves were obtained. Statistical analysis shows that transcripts encoding proteins involved in protein synthesis and in the production of extracellular proteases, cellulases, and xylanases predominate in the infection library. This suggests that the fungus is dependant on the degradation of wheat macromolecular constituents to provide the carbon skeletons and energy for the synthesis of proteins and other components destined for the developing pycnidiospores. © 2007 American Society of Plant Biologists.
Authors & Co-Authors
Hane, James K.
Australia, Perth
Curtin University
Schoch, Conrad L.
United States, Corvallis
Oregon State University
Spatafora, Joseph W.
United States, Corvallis
Oregon State University
Crous, Pedro Willem
Netherlands, Utrecht
Westerdijk Fungal Biodiversity Institute - Knaw
Kodira, Chinnappa Dilip
United States, Cambridge
Broad Institute
Birren, Bruce W.
United States, Cambridge
Broad Institute
Galagan, James E.
United States, Cambridge
Broad Institute
Torriani, Stefano F.F.
Switzerland, Zurich
Eth Zürich
McDonald, Bruce A.
Switzerland, Zurich
Eth Zürich
Oliver, Richard P.
Australia, Perth
Curtin University
Statistics
Citations: 207
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1105/tpc.107.052829
ISSN:
10404651
Research Areas
Cancer
Environmental
Genetics And Genomics
Sexual And Reproductive Health
Study Approach
Quantitative