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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Origin and genetic diversity of Tunisian grapes as revealed by microsatellite markers
Scientia Horticulturae, Volume 120, No. 4, Year 2009
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Description
Ten SSR loci, previously developed for grapevine, were analyzed to evaluate the genetic variability, cultivar relatedness, and parentage in a collection of 61 autochthonous Vitis vinifera cultivars from Tunisia. The number of alleles per locus ranged from 6 to 11, while the number of genotype patterns varied between 10 and 21. The expected heterozygosity varied between 0.621 and 0.855 and the observed heterozygosity was higher than 0.9 at 4 loci (VVMD28, VVMD5, VVIP31 and VVS2) indicating that the SSRs were highly informative. Cluster analysis using unweighted pair group method with arithmetic averaging (UPGMA) suggested 14 groups among studied cultivars and 53 grapevine denominations out of 61 were unequivocally distinguished, with all accessions showing at least one-specific combination of alleles. On the other hand, in order to overcome the existing confusion in Tunisian grapevine nomenclature, of the analyzed homonymous pairs of cultivars, only 'Balta 2' and 'Balta 3' have shown identical allelic profiles, consistent with their being the same genotype. Hence, nomenclature distinction is meaningless and only one denomination should be retained. Due to the high overall power of exclusion (Q) (greater than 99.99%) and to the absence of null alleles, the set of microsatellite loci used is appropriate to determine parentage in Tunisian grapevines beyond any reasonable doubt. The analysis of fingerprints indicated that the Tunisian grape vines have evolved through out crossing between five possible parents: Balta 1, Beldi Baddar, Beldi Rafraf, Beldi Local Rafraf and Khedhiri 3. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Zoghlami, Néjia
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Riahi, Leila
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Laucou, V.
France, Montpellier
Inrae's Occitanie-montpellier Centre
Lacombe, T.
France, Montpellier
Inrae's Occitanie-montpellier Centre
Mliki, A.
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Ghorbel, Abdelwahed
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
This, P.
France, Montpellier
Inrae's Occitanie-montpellier Centre
Statistics
Citations: 33
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.scienta.2008.12.011
ISSN:
03044238
Research Areas
Genetics And Genomics
Study Locations
Tunisia