Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Pearl millet [Pennisetum glaucum (L.) R. Br.] consensus linkage map constructed using four RIL mapping populations and newly developed EST-SSRs
BMC Genomics, Volume 14, No. 1, Article 159, Year 2013
Notification
URL copied to clipboard!
Description
Background: Pearl millet [Pennisetum glaucum (L.) R. Br.] is a widely cultivated drought- and high-temperature tolerant C4 cereal grown under dryland, rainfed and irrigated conditions in drought-prone regions of the tropics and sub-tropics of Africa, South Asia and the Americas. It is considered an orphan crop with relatively few genomic and genetic resources. This study was undertaken to increase the EST-based microsatellite marker and genetic resources for this crop to facilitate marker-assisted breeding.Results: Newly developed EST-SSR markers (99), along with previously mapped EST-SSR (17), genomic SSR (53) and STS (2) markers, were used to construct linkage maps of four F7 recombinant inbred populations (RIP) based on crosses ICMB 841-P3 × 863B-P2 (RIP A), H 77/833-2 × PRLT 2/89-33 (RIP B), 81B-P6 × ICMP 451-P8 (RIP C) and PT 732B-P2 × P1449-2-P1 (RIP D). Mapped loci numbers were greatest for RIP A (104), followed by RIP B (78), RIP C (64) and RIP D (59). Total map lengths (Haldane) were 615 cM, 690 cM, 428 cM and 276 cM, respectively. A total of 176 loci detected by 171 primer pairs were mapped among the four crosses. A consensus map of 174 loci (899 cM) detected by 169 primer pairs was constructed using MergeMap to integrate the individual linkage maps. Locus order in the consensus map was well conserved for nearly all linkage groups. Eighty-nine EST-SSR marker loci from this consensus map had significant BLAST hits (top hits with e-value ≤ 1E-10) on the genome sequences of rice, foxtail millet, sorghum, maize and Brachypodium with 35, 88, 58, 48 and 38 loci, respectively.Conclusion: The consensus map developed in the present study contains the largest set of mapped SSRs reported to date for pearl millet, and represents a major consolidation of existing pearl millet genetic mapping information. This study increased numbers of mapped pearl millet SSR markers by >50%, filling important gaps in previously published SSR-based linkage maps for this species and will greatly facilitate SSR-based QTL mapping and applied marker-assisted selection programs. © 2013 Rajaram et al.; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S1.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S2.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S3.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S4.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S5.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S6.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S7.pptx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S8.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3606598/bin/1471-2164-14-159-S9.pptx
Authors & Co-Authors
Rajaram, Vengaldas
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
India, Hyderabad
Jawaharlal Nehru Technological University Hyderabad
Thirunavukkarasu, Nepolean
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
India, New Delhi
Icar - Indian Agricultural Research Institute, new Delhi
Senthilvel, Senapathy
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
India, Hyderabad
Icar - Indian Institute of Oilseeds Research, Hyderabad
Varshney, Rajeev Kumar
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Vadez, Vincent
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Srivastava, Rakesh Kumar
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Shah, Trushar M.
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Ambawat, Supriya
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
India, Hisar
Ccs Haryana Agricultural University
Kumar, Suśhil
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
India, Anand
Anand Agricultural University
Ramana Kumari, Basava
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
BhanuPrakash, Amindala
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Lakshmi Narasu, Mangamoori
India, Hyderabad
Jawaharlal Nehru Technological University Hyderabad
Riera-Lizarazu, Oscar
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
United States, Corvallis
Oregon State University
Hash, Charles Tom
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Niger, Niamey
International Crops Research Institute for the Semi-arid Tropics Sahelian Center Niamey
Statistics
Citations: 14
Authors: 14
Affiliations: 8
Identifiers
Doi:
10.1186/1471-2164-14-159
e-ISSN:
14712164
Research Areas
Genetics And Genomics