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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Gonadotropin-releasing hormone (GnRH)-binding sites in human breast cancer cell lines and inhibitory effects of GnRH antagonists
Journal of Clinical Endocrinology and Metabolism, Volume 64, No. 3, Year 1987
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Description
GnRH-binding sites have previously been described in human breast tumors, and a GnRH agonist has been shown to inhibit growth of the MCF-7 human breast cancer cell line. We have investigated the presence of GnRH-binding sites in ZR-75-1, MDA-MB-231, Sk Br 3, MDA-MB-157, and MCF-7 human breast cancer cell lines and the effect of GnRH analogs on the incorporation of [3H]thymidine and 14C-labeled amino acids into DNA and protein. Specific GnRH-binding sites were present in membrane preparations of all five human breast carcinoma cell lines. Studies in three cell lines indicated low affinity (Kd, 1.6-3.0 × 10-6 M) GnRH binding similar to that reported in human placenta and corpus luteum. In contrast, human pituitary GnRH receptors were of high affinity (Kd, 4.8 × 10-9 M). Breast carcinoma cell GnRH-binding sites also differed from the pituitary receptor in their inability to discriminate between GnRH and superactive analogs. Binding of a [125I] GnRH analog to ZR-75-1 breast cancer cells and pituitary membranes was affected similarly by various cations. GnRH antagonists rapidly inhibited [3H]thymidine incorporation into DNA (within 3 hr), and this effect was reversible. GnRH antagonists also inhibited cell growth, but only after 6 days. GnRH agonists did not alter either thymidine incorporation or growth. The present observations of low affinity GnRH-binding sites in breast cancer cell lines and inhibitory effects of GnRH antagonists point to the possibility of an autocrine regulatory role of GnRH-like peptides in mammary cells. © 1987 by The Endocrine Society.
Authors & Co-Authors
Eidne, Karin Ann
South Africa, Cape Town
Faculty of Health Sciences
United Kingdom, Edinburgh
Mrc Centre for Reproductive Health
Flanagan, Colleen A.
South Africa, Cape Town
Faculty of Health Sciences
Harris, N. S.
South Africa, Cape Town
Faculty of Health Sciences
Millar, Robert P.
South Africa, Cape Town
Faculty of Health Sciences
Statistics
Citations: 218
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1210/jcem-64-3-425
ISSN:
0021972X
e-ISSN:
19457197
Research Areas
Cancer
Genetics And Genomics