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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Partial characterization of bacteriocin AMA-K, produced by Lactobacillus plantarum AMA-K isolated from naturally fermented milk from Zimbabwe
Food Control, Volume 18, No. 6, Year 2007
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Description
Strain AMA-K, isolated from naturally fermented milk produced in Gwanda, Kafusi area, Zimbabwe, was identified as Lactobacillus plantarum based on sugar fermentation reactions (API 50 CHL) and PCR with species-specific primers. The cell-free supernatant containing bacteriocin AMA-K inhibited the growth of Listeria innocua and Enterococcus faecalis. Based on tricine-SDS-PAGE, bacteriocin AMA-K is 2.9 kDa in size. Activity levels of 12 800 AU/ml was recorded in MRS broth at 30 °C and 37 °C. Complete inactivation or significant reduction in bacteriocin activity was observed after treatment with proteolytic enzymes, but not with catalase and α-amylase. Bacteriocin activity was not destroyed after treatment with SDS, Tween 20, Tween 80, urea, triton X-100 or EDTA. Loss of activity was recorded after treatment with Triton X-114. Bacteriocin AMA-K remained stable after 2 h of incubation at pH 2.0-12.0 and at 100 °C, respectively. The bacteriocin not adheres to the surface of the producer cell and has a bacteriolytic mode of action. Bacteriocin production is stimulated by the presence of Listeria innocua. Lactobacillus plantarum AMA-K grows in milk, but produces only 800 AU bacteriocin per ml after 24 h. © 2006 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Todorov, S. D.
South Africa, Stellenbosch
Stellenbosch University
Nyati, Hilda
Zimbabwe, Bulawayo
National University of Science and Technology Bulawayo
Meincken, Martina
South Africa, Stellenbosch
Stellenbosch University
Dicks, Leon Milner Theodore
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 107
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.foodcont.2006.03.003
ISSN:
09567135
Study Locations
Zimbabwe