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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Isolation and selection of Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish
Journal of Applied Microbiology, Volume 103, No. 5, Year 2007
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Description
Aims: To isolate, select and evaluate Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. Methods and Results: Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibition of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a preliminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products. © 2007 CSIR.
Authors & Co-Authors
Lalloo, Rajesh
South Africa, Modderfontein
Csir Biosciences
Ramchuran, Santosh O.
South Africa, Modderfontein
Csir Biosciences
Ramduth, Danni
South Africa, Modderfontein
Csir Biosciences
Görgens, Johann Ferdinand
South Africa, Stellenbosch
Stellenbosch University
Gardiner, Neil S.
South Africa, Modderfontein
Csir Biosciences
Statistics
Citations: 122
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1111/j.1365-2672.2007.03360.x
ISSN:
13645072
e-ISSN:
13652672
Research Areas
Environmental