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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Successful implementation of a low-cost method for enumerating CD4
+
T lymphocytes in resource-limited settings: The ANRS 12-26 study
AIDS, Volume 17, No. 15, Year 2003
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Description
Objective: To evaluate the feasibility and the relevance of the implementation of an alternative technique to flow cytometry (FC) for enumerating CD4 T cells (Dynabeads; Dynal Biotech, Oslo, Norway), based on quantifying CD4 T cells by epifluorescent microscopy following their isolation using anti-CD4 monoclonal antibody-coated magnetic beads. Design: International multi-center study. Five consecutive runs of dual CD4 T-lymphocyte enumeration by both techniques in six sites in five countries of West Africa. Methods: A total of 657 pairs of values of CD4 cell counts were generated by 43 technicians by both FC (TruCount; Becton Dickinson Immunocytometry Systems, San Jose, California, USA) and Dynabeads from blood samples obtained from 301 HIV-infected patients, seen in one (n = 112), two (n = 61), three (n = 75), four (n = 40) or five (n = 13) occasions. Results: The correlation coefficient between the results of the two techniques was 0.89. The overall systematic difference between Dynabeads and FC was -16 x 106 cells/l (P < 10-4). The median difference was insignificant (+7.5 cells) for CD4 cell counts below 200 x 106 cells/l and increased with CD4 levels. Patients were consistently classified at the threshold of 200 x 106 cells/l by both methods in 88.7% of cases. Among the 74 discrepant pairs of values, only 31 (4.7%) exhibited a difference of more than 100 x 106 cells/l. Conclusions: Results from Dynabeads and FC were highly correlated. The ability of the alternative method to consistently classify results in agreement with FC, at thresholds of CD4 cell counts relevant for clinical care, was high. The implementation of this low-cost method was easy and successful in the West African context. © 2003 Lippincott Williams & Wilkins.
Authors & Co-Authors
Diagbouga, Serge P.
Burkina Faso, Ouagadougou
Centre Muraz
Chazallon, Corine
France, Paris
Inserm
Kazatchkine, Michel Daniel
France, Paris
Hôpital Européen Georges-pompidou
van de Perre, Philippe
France, Montpellier
Hopital Arnaud de Villeneuve
Inwoley, André
Cote D'ivoire, Abidjan
Cedres
M'Boup, Souleymane
Senegal, Dakar
Centre Hospitalier Universitaire Aristide le Dantec de Dakar
David, Mireille Prince
Togo, Lome
Centre Hospitalier Universitaire Tokoin
Ténin, Aoua Thiéro
Mali, Bamako
Institut National de Recherche en Sante Publique Mali
Soudré, Robert B.
Ouagadougou
Faculté de Médecine de Ouagadougou
Aboulker, Jean Pierre
France, Paris
Inserm
Weiss, Laurence L.
France, Paris
Hôpital Européen Georges-pompidou
Statistics
Citations: 72
Authors: 11
Affiliations: 9
Identifiers
Doi:
10.1097/00002030-200310170-00008
Research Areas
Infectious Diseases
Study Locations
Multi-countries