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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
L-asparaginase production by Pseudomonas aeruginosa in solid-state culture: Evaluation and optimization of culture conditions using factorial designs
Process Biochemistry, Volume 38, No. 1, Year 2002
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Description
Statistically-based experimental designs were applied to optimize a solid-state fermentation for the production of L-asparaginase by Pseudomonas aeruginosa 50071. Fifteen culture conditions were examined for their significance on enzyme production and specific activity using Plackett-Burman factorial design. pH, casein hydrolysate and corn steep liquor were the most significant factors improving enzyme production process. The second optimization step was to identify optimal values of the three factors that bringing about maximum L-asparaginase activity, using Box-Behnken design. Maximal enzyme activity (142.8 IU) has been detected under the following conditions, pH 7.9; casein hydrolysate, 3.11%; and corn steep liquor, 3.68% which is more than five folds the activity in basal medium. A verification experiment was carried out to examine model validation and revealed more than 99% validity. © 2002 Elsevier Science Ltd. All rights reserved.
Authors & Co-Authors
Abdel-Fattah, Yasser Refaat
Egypt, New Borg el Arab
Genetic Engineering and Biotechnology Research Institute
Olama, Zakia Ahmed
Egypt, Alexandria
Faculty of Agriculture Sababasha
Statistics
Citations: 179
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1016/S0032-9592(02)00067-5
ISSN:
13595113
Study Approach
Quantitative