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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Antibacterial and leishmanicidal activities of temporin-SHd, a 17-residue long membrane-damaging peptide
Biochimie, Volume 95, No. 2, Year 2013
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Description
Temporins are a family of short antimicrobial peptides (8-17 residues) that mostly show potent activity against Gram-positive bacteria. Herein, we demonstrate that temporin-SHd, a 17-residue peptide with a net charge of +2 (FLPAALAGIGGILGKLFamide), expressed a broad spectrum of antimicrobial activity. This peptide displayed potent antibacterial activities against Gram-negative and Gram-positive bacteria, including multi-drug resistant Staphylococcus aureus strains, as well as antiparasitic activity against promastigote and the intracellular stage (amastigote) of Leishmania infantum, at concentration not toxic for the macrophages. Temporin-SHd that is structured in a non-amphipathic α-helix in anionic membrane-mimetic environments, strongly and selectively perturbs anionic bilayer membranes by interacting with the polar head groups and acyl region of the phospholipids, with formation of regions of two coexisting phases: one phase rich in peptide and the other lipid-rich. The disruption of lipid packing within the bilayer may lead to the formation of transient pores and membrane permeation/disruption once a threshold peptide accumulation is reached. To our knowledge, Temporin-SHd represents the first known 17-residue long temporin expressing such broad spectrum of antimicrobial activity including members of the trypanosomatidae family. Additionally, since only a few shorter members (13 residues) of the temporin family are known to display antileishmanial activity (temporins-TA, -TB and -SHa), SHd is an interesting tool to analyze the antiparasitic mechanism of action of temporins. © 2012 Elsevier Masson SAS. All rights reserved.
Authors & Co-Authors
Abbassi, Feten
France, Paris
Sorbonne Université
Tunisia, Beja
Institut Superieur de Biotechnologie de Beja
Raja, Zahid
France, Paris
Sorbonne Université
Oury, Bruno
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Gazanion, Elodie
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Canada, Quebec
Centre de Recherche en Infectiologie
Piesse, Christophe
France, Paris
Sorbonne Université
Sereno, Denis
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Nicolas, Pierre
France, Paris
Sorbonne Université
Foulon, Thierry
France, Paris
Sorbonne Université
Ladram, Ali
France, Paris
Sorbonne Université
Tunisia, Beja
Institut Superieur de Biotechnologie de Beja
Statistics
Citations: 41
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1016/j.biochi.2012.10.015
ISSN:
03009084
e-ISSN:
61831638
Research Areas
Environmental
Infectious Diseases