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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Modulation of hepatic drug metabolizing enzymes by dietary doses of thymoquinone in female New Zealand white rabbits
Phytotherapy Research, Volume 26, No. 11, Year 2012
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Description
Herbal medicines can affect drug metabolizing enzymes. Therefore the effect of thymoquinone (TQ), the active ingredient of black seeds, was examined on rabbit liver drug metabolizing enzymes. Two groups of New Zealand female rabbits received TQ at 10 and 20 mg/kg/day orally and a control group of six animals each were killed after 8 weeks. Blood and livers were harvested and the activity of cytochrome P450 (CYP) and phase II enzymes in the microsomal and cytosolic preparations were measured by HPLC and ELISA methods. The liver enzymes ALT/AST and albumin were similar in the three groups. CYP1A2, CYP3A4, but not CYP2E1, were significantly diminished by TQ treatment. Of the phase II enzymes, glutathione-S-transferase (GST) and glutathione peroxidase (GPx) were significantly induced by the high TQ dose, while the total glutathione levels were unaffected. Glutathione reductase (GR), on the other hand, was significantly induced in the two experimental groups. Thymoquinone has differential effects on CYP and phase II enzymes. Inhibition of some CYP enzyme activities may lead to a hazardous herb-drug interaction. Induction of GR activity may explain the salutatory effect of the black seeds in inhibiting the generation of bioactive metabolites known to promote carcinogenesis and oxidative cell damage. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.
Authors & Co-Authors
Elbarbry, Fawzy A.
United States, Forest Grove
Pacific University
Ragheb, Ahmed
Egypt, Shibin el Kom
Menoufia University Faculty of Medicine
Marfleet, Travis
Canada, Saskatoon
University of Saskatchewan, College of Medicine
Shoker, Ahmed Said
Canada, Saskatoon
Royal University Hospital
Statistics
Citations: 37
Authors: 4
Affiliations: 4
Identifiers
Doi:
10.1002/ptr.4628
ISSN:
0951418X
e-ISSN:
10991573
Research Areas
Cancer
Study Design
Randomised Control Trial
Participants Gender
Female