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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
First detection of Leishmania major DNA in Sergentomyia (Spelaeomyia) darlingi from cutaneous leishmaniasis foci in Mali
PLoS ONE, Volume 7, No. 1, Article e28266, Year 2012
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Description
Background: Leishmania major complex is the main causative agent of zoonotic cutaneous leishmaniasis (ZCL) in the Old World. Phlebotomus papatasi and Phlebotomus duboscqi are recognized vectors of L. major complex in Northern and Southern Sahara, respectively. In Mali, ZCL due to L. major is an emerging public health problem, with several cases reported from different parts of the country. The main objective of the present study was to identify the vectors of Leishmania major in the Bandiagara area, in Mali. Methodology/Principal Findings: An entomological survey was carried out in the ZCL foci of Bandiagara area. Sandflies were collected using CDC miniature light traps and sticky papers. In the field, live female Phlebotomine sandflies were identified and examined for the presence of promastigotes. The remaining sandflies were identified morphologically and tested for Leishmania by PCR in the ITS2 gene. The source of blood meal of the engorged females was determined using the cyt-b sequence. Out of the 3,259 collected sandflies, 1,324 were identified morphologically, and consisted of 20 species, of which four belonged to the genus Phlebotomus and 16 to the genus Sergentomyia. Leishmania major DNA was detected by PCR in 7 of the 446 females (1.6%), specifically 2 out of 115 Phlebotomus duboscqi specimens, and 5 from 198 Sergentomyia darlingi specimens. Human DNA was detected in one blood-fed female S. darlingi positive for L. major DNA. Conclusion: Our data suggest the possible involvement of P. duboscqi and potentially S. darlingi in the transmission of ZCL in Mali. © 2012 Berdjane-Brouk et al.
Authors & Co-Authors
Berdjane-Brouk, Zohra
France, Villetaneuse
University Sorbonne Paris Nord
France, Marseille
Aix Marseille Université
Koné, Abdoulaye Kassoum
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Djimde, Abdoulaye A.
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Charrel, Remi N.
France, Marseille
Aix Marseille Université
Ravel, Christophe
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Delaunay, Pascal
France, Nice
Centre Hospitalier Universitaire de Nice
Del-Giudice, Pascal D.
France
Hôpital Bonnet 83700 Fréjus
Diarra, Adama Zan
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Doumbo, Siala
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Goita, S. M.
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Thera, Mahamadou Ali
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Depaquit, Jérôme
France, Reims
Université de Reims Champagne-ardenne
Marty, Pierre M.
France, Nice
Centre Hospitalier Universitaire de Nice
Doumbo, Ogobara K.
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Izri, Arezki
France, Villetaneuse
University Sorbonne Paris Nord
France, Marseille
Aix Marseille Université
Statistics
Citations: 72
Authors: 15
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pone.0028266
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Mali
Participants Gender
Female