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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
HIV-1 superinfections in a cohort of commercial sex workers in Burkina Faso as assessed by an autologous heteroduplex mobility procedure
AIDS, Volume 18, No. 12, Year 2004
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Description
Objective: To describe and evaluate a simple procedure to identify HIV-1 co- or super-infections based on a heteroduplex mobility assay (HMA). Methods: To identify heteroduplexes corresponding to divergent viral populations in a the same individual, HMA was applied to single DNA samples from each subject in a prospective cohort of commercial sex workers (CSW) in Bobo-Dioulasso, Burkina Faso. After denaturation and renaturation of env DNA amplicons, hybridized DNA was separated by electrophoresis through polyacrylamide gel. HIV-1 co-infections were suspected by slow migration of heteroduplex, at a level comparable to that of mixed reference strains. Following electrophoresis, DNA in bands representing heteroduplex was extracted and cloned in a plasmid vector; identification of phylogenetically distinct clones was confirmed by sequencing divergent clones identified through a second HMA step of a pair of two mixed clones. Results: Among 147 HIV-1 infected CSW, four had an autologous HMA profile comparable to low mobility of hybridized DNA from mixed reference strains representing most frequent HIV-1 clades and circulating recombinant forms (CRF) prevalent in Burkina Faso. In two of them, two phylogenetically distinct HIV-1 populations were coexisting. The first woman presented with a CRF02-AG and CRF06-cpx co-infection, and the second with a CRF02-AG and a divergent virus co-infection not significantly related to any other known subtypes. In both women, retrospective analyses of stored samples by the same HMA procedure showed acquisition of a second virus concomitent with an increasing plasma HIV RNA. Conclusions: Autologous HMA procedure followed by acrylamide extraction of heteroduplexes allowed identifying HIV-1 co- and super-infections in a large cohort study. HIV-1 super-infection is not an uncommon phenomenon. © 2004 Lippincott Williams & Wilkins.
Authors & Co-Authors
Manigart, Olivier
Burkina Faso, Ouagadougou
Centre Muraz
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
Luxembourg, Strassen
Luxembourg Institute of Health
Courgnaud, Valérie
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
Sanou, Oumarou
Burkina Faso, Ouagadougou
Centre Muraz
Valéa, Diane C.
Burkina Faso, Ouagadougou
Centre Muraz
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
Nagot, Nicolas
Burkina Faso, Ouagadougou
Centre Muraz
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
Méda, Nicolas Yelbomkan
Burkina Faso, Ouagadougou
Centre Muraz
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
Delaporte, Éric
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
Peeters, Martine F.
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
van de Perre, Philippe
France, Montpellier
Recherches Translationnelles Sur le Vih et Les Maladies Infectieuses
France, Montpellier
Hopital Arnaud de Villeneuve
Statistics
Citations: 57
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1097/01.aids.0000131333.30548.db
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cohort Study
Study Approach
Quantitative
Study Locations
Burkina Faso
Participants Gender
Female