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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Effects of Extraction Solvents and Provenances on Phenolic Contents and Antioxidant Activities of Cumin (Cuminum cyminum L.) Seeds
Food and Bioprocess Technology, Volume 5, No. 7, Year 2012
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Description
Polyphenol contents and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity of cumin (Cuminum cyminum L.) seed extracts were compared depending on their geographical origin (Tunisia and India: TCS and ICS, respectively) and the extraction solvent polarity. The β-carotene bleaching assay, the chelating ability and the reducing power of the most promising solvent extracts were also assessed. In addition, TCS and ICS extracts were acid-hydrolyzed and the phenolics identified by reversed-phase high-performance liquid chromatography (HPLC). Seed phenolic contents and antioxidant activity appeared to be accession and solvent dependent. Extraction with 80% acetone led to the highest polyphenol (18.60 and 16.50 mg gallic acid equivalents (GAE)/g dry weight (DW)), flavonoid (5.91 and 4.99 mg catechin equivalents (CE)/g DW) and tannin (83.23 and 80.23 mg CE/g DW) contents, respectively for TCS and ICS. DPPH scavenging activity, β-carotene bleaching assay, chelating ability and reducing power were maximal in 80% acetone for both TCS and ICS. HPLC analysis revealed several phenolic compounds in C. cyminum seeds, with p-coumaric (4.83 and 2.33 mg/g DW), trans-2-dihydrocinnamic (1.09 and 1.20 mg/g DW) and rosmarinic (0.70 and 1.04 mg/g DW) acids as major phenolics in TCS and ICS, respectively. Thus, phenolic composition of cumin seeds is also origin dependent. Taken together, our findings indicate that cumin might constitute a rich and novel source of natural antioxidants as food additives in food industry and that acetone 80% would be the most appropriate solvent for seed extraction. © 2011 Springer Science + Business Media, LLC.
Authors & Co-Authors
Bettaieb Rebey, Iness
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Bourgou, Soumaya
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Ben Slimene, Imen
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Karoui, Iness Jabri
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Hamrouni, Ibtissem
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Msaada, Kamel
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Limam, Férid
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Marzouk, Brahim
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Statistics
Citations: 93
Authors: 8
Affiliations: 1
Identifiers
Doi:
10.1007/s11947-011-0625-4
e-ISSN:
19355149
Research Areas
Food Security
Study Locations
Tunisia