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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Molecular cytogenetic characterization of Philadelphia-negative rearrangements in chronic myeloid leukemia patients
Journal of Cancer Research and Clinical Oncology, Volume 137, No. 9, Year 2011
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Description
Background: The BCR/ABL gene rearrangement is generated by a reciprocal translocation t(9;22)(q34;q11) in chronic myeloid leukemia (CML) patients. In most cases, it is cytogenetically visualized by the Philadelphia (Ph) chromosome. About 5-10% of CML patients lack cytogenetic evidence of the Ph translocation but show BCR/ABL fusion by fluorescence in situ hybridization (FISH) or reverse transcriptase-polymerase chain reaction (RT-PCR). Deletions around the breakpoints on derivative chromosome 9 including 5'ABL and 3'BCR sequences occur in 10-15% of Ph-positive CML patients and are thought to have prognostic significance. Methods: We explored cryptic rearrangements involving chromosomes 9 and 22 in 3 CML patients with an apparently normal bone marrow karyotypes using multiplex RT-PCR and FISH with commercial and home-brew probes. Results: The BCR/ABL fusion transcripts were detected by RT-PCR. Using commercial FISH probes, the BCR/ABL fusion gene was found on chromosome 22 in two patients and on chromosome 9 in one patient. Consecutive FISH assays clarified the mechanism of the masked Ph chromosome: in the 3 patients, Ph rearrangement resulted from double mechanism consisting in standard translocation t(9;22)(q34;q11) followed by a second reversed translocation t(9;22)(q34;q11). One patient achieved major cytogenetic response after 6 months of imatinib therapy, and one patient had successful bone marrow transplant. Conclusions: In this study, we have characterized three Ph-negative CML patients with cryptic BCR/ABL rearrangement generated after an uncommon mechanism involving two sequential translocations and confirm that the BCR/ABL hybrid gene may be located on other sites than 22q11. Ph-negative CML patients with BCR/ABL fusion gene have the same prognosis as patients with classical t(9;22). © Springer-Verlag 2011.
Authors & Co-Authors
Bennour, Ayda
Tunisia, Sousse
Hopital Farhat Hached Sousse
Bellâaj, Hatem
Tunisia, Sfax
Chu Hedi-chaker
Ben Youssef, Yosra
Tunisia, Sousse
Hopital Farhat Hached Sousse
Elloumi, Moez
Tunisia, Sfax
Chu Hedi-chaker
Khélif, Abderrahim
Tunisia, Sousse
Hopital Farhat Hached Sousse
Saâd, Ali
Tunisia, Sousse
Hopital Farhat Hached Sousse
Sennana, Halima
Tunisia, Sousse
Hopital Farhat Hached Sousse
Statistics
Citations: 16
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1007/s00432-011-1002-4
ISSN:
01715216
e-ISSN:
14321335
Research Areas
Genetics And Genomics
Health System And Policy