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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
The role of avian reoviruses in turkey tenosynovitis/arthritis
Avian Pathology, Volume 43, No. 4, Year 2014
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Description
Turkey arthritis reovirus (TARV) has been isolated from the gastrocnemius tendons and tibiotarsal joint fluid of lame male turkeys >12 weeks old in the Midwest. Two experiments were conducted to compare the pathogenicity in turkeys of three TARVs (TARV-MN2, TARV-MN4 and TARV-O'Neil), one turkey enteric reovirus (TERV strain MN1) and one chicken arthritis reovirus (CARV strain MN1). Two hundred microlitres of virus were inoculated by the oral, intratracheal, or footpad route into 6-day-old poults placed in isolator units. Poults were necropsied at 1 and 4 weeks post infection in Experiment 1, and at 2 and 4 weeks post infection in Experiment 2. Reovirus was detected by reverse transcription-polymerase chain reaction and virus isolation in tendons of TARV-inoculated poults at 1, 2 and 4 weeks post infection. TARV-O'Neil and TARV-MN2 were detected in tendons of sentinal birds at 1 and 4 weeks and 1 week p.i., respectively. In general, TARVs produced lymphocytic tenosynovitis of the gastrocnemius and digital flexor tendon sheaths without inflammation of the tendons proper. In Experiment 1, poults inoculated with TARV-MN2 and TARV-O'Neil had significantly higher gastrocnemius tendon inflammation scores, as determined by histology, than those inoculated with TERV-MN1 or CARV-MN1. In Experiment 2, poults inoculated with TARV-MN2 and TARV-O'Neil had significantly higher gastrocnemius tendon inflammation scores than those inoculated with TARV-MN4 and virus-free medium (negative control group). Koch's postulates was fulfilled when TARV-MN2 and TARV-O'Neil were re-isolated from tendons of poults that had originally been challenged with either of these viruses. Results of these experiments indicate that TARVs have a unique ability to induce gastrocnemius tenosynovitis in turkeys and that administration of TARV-O'Neil through the oral or intratracheal route is a reproducible model to study pathogenesis of TARV infection. © 2014 Houghton Trust Ltd.
Authors & Co-Authors
Sharafeldin, Tamer A.
United States, Minneapolis
University of Minnesota Twin Cities
Egypt, Zagazig
Zagazig University
Mor, Sunil Kumar
United States, Minneapolis
University of Minnesota Twin Cities
Bekele, Aschalew Z.
United States, Minneapolis
University of Minnesota Twin Cities
Verma, Harsha
United States, Minneapolis
University of Minnesota Twin Cities
Goyal, Sagar Mal
United States, Minneapolis
University of Minnesota Twin Cities
Porter, Robert E.
United States, Minneapolis
University of Minnesota Twin Cities
Statistics
Citations: 29
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1080/03079457.2014.940496
ISSN:
03079457
e-ISSN:
14653338
Research Areas
Health System And Policy
Study Design
Randomised Control Trial
Study Approach
Quantitative
Participants Gender
Male