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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
veterinary
Subpopulations of bovine WC1
+
γδ T cells rather than CD4
+
CD25
high
Foxp3
+
T cells act as immune regulatory cells ex vivo
Veterinary Research, Volume 40, No. 1, Year 2009
Notification
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Description
Regulatory T cells (Treg) are regarded essential components for maintenance of immune homeostasis. Especially CD4+CD25high T cells are considered to be important regulators of immune reactivity. In humans and rodents these natural Treg are characterized by their anergic nature, defined as a non-proliferative state, suppressive function and expression of Foxp3. In this study the potential functional role of flowcytometry-sorted bovine white blood cell populations, including CD4+CD25high T cells and γδ T cell subpopulations, as distinct ex vivo regulatory cells was assessed in co-culture suppression assays. Our findings revealed that despite the existence of a distinct bovine CD4+CD25high T cell population, which showed Foxp3 transcription/expression, natural regulatory activity did not reside in this cell population. In bovine co-culture suppression assays these cells were neither anergic nor suppressive. Subsequently, the following cell populations were tested functionally for regulatory activity: CD4+CD25low T cells, WC1+, WC1.1+ and WC1.2+ γδ T cells, NK cells, CD8+ T cells and CD14+ monocytes. Only the WC1.1 + and WC1.2+ γδ T cells and CD14+ monocytes proved to act as regulatory cells in cattle, which was supported by the fact that these regulatory cells showed IL-10 transcription/expression. In conclusion, our data provide first evidence that cattle CD4+CD25 highFoxp3+ and CD4+CD25low T cells do not function as Treg ex vivo. The bovine Treg function appears to reside in the γδ T cell population, more precisely in the WC1.1+ and the WC1.2+ subpopulation, major populations present in blood of cattle in contrast to non-ruminant species. © 2008 INRA EDP Sciences.
Authors & Co-Authors
Hoek, Aad C.
Netherlands, Utrecht
Universiteit Utrecht
Rutten, Victor P.M.G.
Netherlands, Utrecht
Universiteit Utrecht
South Africa, Pretoria
University of Pretoria
Kool, Jolanda
Netherlands, Utrecht
Universiteit Utrecht
Arkesteijn, Ger J.A.
Netherlands, Utrecht
Universiteit Utrecht
Bouwstra, Ruth J.
Netherlands, Utrecht
Universiteit Utrecht
Van Rhijn, Ildiko
Netherlands, Utrecht
Universiteit Utrecht
Koets, Ad P.
Netherlands, Utrecht
Universiteit Utrecht
Statistics
Citations: 120
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1051/vetres:2008044
ISSN:
09284249
e-ISSN:
12979716
Study Design
Cross Sectional Study