Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Methods for Identifying Neisseria meningitidis Carriers: A Multi-Center Study in the African Meningitis Belt
PLoS ONE, Volume 8, No. 10, Article e78336, Year 2013
Notification
URL copied to clipboard!
Description
Objective:Detection of meningococcal carriers is key to understanding the epidemiology of Neisseria meningitidis, yet no gold standard has been established. Here, we directly compare two methods for collecting pharyngeal swabs to identify meningococcal carriers.Methods:We conducted cross-sectional surveys of schoolchildren at multiple sites in Africa to compare swabbing the posterior pharynx behind the uvula (U) to swabbing the posterior pharynx behind the uvula plus one tonsil (T). Swabs were cultured immediately and analyzed using molecular methods.Results:One thousand and six paired swab samples collected from schoolchildren in four countries were analyzed. Prevalence of meningococcal carriage was 6.9% (95% CI: 5.4-8.6%) based on the results from both swabs, but the observed prevalence was lower based on one swab type alone. Prevalence based on the T swab or the U swab alone was similar (5.2% (95% CI: 3.8-6.7%) versus 4.9% (95% CI: 3.6-6.4%) respectively (p=0.6)). The concordance between the two methods was 96.3% and the kappa was 0.61 (95% CI: 0.50-0.73), indicating good agreement.Conclusions:These two commonly used methods for collecting pharyngeal swabs provide consistent estimates of the prevalence of carriage, but both methods misclassified carriers to some degree, leading to underestimates of the prevalence. © 2013 Basta et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3806823/bin/pone.0078336.s001.docx
Authors & Co-Authors
Basta, Nicole E.
Unknown Affiliation
Stuart, James Mac Naughton
Unknown Affiliation
Nascimento, M.
Unknown Affiliation
Manigart, Olivier
Unknown Affiliation
Trotter, Caroline
Unknown Affiliation
Hassan-King, Musa K.A.
Unknown Affiliation
Chandramohan, Daniel
Unknown Affiliation
Sow, Samba Ousmane
Unknown Affiliation
Berthe, Abdoulaye
Unknown Affiliation
Bedru, Ahmed
Unknown Affiliation
Tekletsion, Yenenesh K.
Unknown Affiliation
Collard, Jean Marc
Unknown Affiliation
Jusot, Jean François
Unknown Affiliation
Diallo, Aldiouma M.
Unknown Affiliation
Basséne, Hubert
Unknown Affiliation
Daugla, Doumagoum Moto
Unknown Affiliation
Gamougam, Kadidja
Unknown Affiliation
Hodgson, Abraham V.O.
Unknown Affiliation
Forgor, Abudulai Adams
Unknown Affiliation
Omotara, Babatunji Abayomi
Unknown Affiliation
Gadzama, Galadima Bala
Unknown Affiliation
Watkins, Eleanor Rose
Unknown Affiliation
Rebbetts, Lisa S.
Unknown Affiliation
Diallo, Kanny
Unknown Affiliation
Weiss, Noel Scott
Unknown Affiliation
Halloran, Mary Elizabeth
Unknown Affiliation
Maiden, Martin Christopher James
Unknown Affiliation
Greenwood, Brian M.
Unknown Affiliation
Statistics
Citations: 28
Authors: 28
Affiliations: 16
Identifiers
Doi:
10.1371/journal.pone.0078336
e-ISSN:
19326203
Study Design
Cross Sectional Study