Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Standard Conditions of Virus Isolation Reveal Biological Variability of HIV Type 1 in Different Regions of the World
AIDS Research and Human Retroviruses, Volume 10, No. 11, Year 1994
Notification
URL copied to clipboard!
Description
HIV-1 isolates were obtained from four countries within the framework of the WHO Network for HIV Isolation and Characterization. The use of standard HIV isolation procedures allowed us to compare the biological properties of 126 HIV-1 isolates spanning five genetic subtypes. In primary isolation cultures, viruses from Uganda and Brazil appeared early and replicated without delay, whereas the replication of Thai viruses was delayed by several weeks. Regardless of genetic subtype or country of origin, blood samples collected more than 2 years after seroconversion yielded virus that replicated efficiently in the primary isolation cultures. None of the isolates obtained from Thailand or Rwanda replicated in cell lines, whereas 5 of the 13 Brazilian isolates and 7 of the 11 Ugandan isolates replicated and induced syncytia in MT-2 cells. As expected for virus isolates obtained early in HIV-1 infection (within 2 years of seroconversion), all viruses from Brazil, Rwanda, and Thailand showed a slow/low replicative pattern. For the Ugandan samples, the time from seroconversion was known precisely for a few of the samples and only in one case was less than 2 years. This may explain why the five viruses that were able to replicate in all cell lines, and thus classified as rapid/high, were of Ugandan origin. Viruses able to induce syncytia in MT-2 cells, also induced syncytia in PBMC. However, 8 slow/low viruses (out of 27) gave discordant results, inducing syncytia in PBMC but not in MT-2 cells. Furthermore, using syncytium induction as a marker, changes in virus populations during early in vitro passage in PBMC could be observed. The results indicate that biological variation is a general property of HIV-1 in different regions of the world. Moreover, the time from HIV-1 infection, rather than genetic subtype, seems to be linked to viral phenotype. © 1994, Mary Ann Liebert, Inc. All rights reserved.
Authors & Co-Authors
Rübsamen-Waigmann, Helga
Germany, Frankfurt am Main
Georg-speyer-haus
von Briesen, Hagen
Germany, Frankfurt am Main
Georg-speyer-haus
Holmes, Harvey C.
United Kingdom, Hertfordshire
National Institute for Biological Standards and Control
Björndal, Åsa
Sweden, Solna
Department of Microbiology, Tumor and Cell Biology
Korber, Bette T.
United States, Los Alamos
Los Alamos National Laboratory
Esser, R.
Germany, Frankfurt am Main
Georg-speyer-haus
Ranjbar, S.
United Kingdom, Hertfordshire
National Institute for Biological Standards and Control
Tomlinson, P.
United Kingdom, Hertfordshire
National Institute for Biological Standards and Control
Galvão-Castro, Bernardo
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Karita, Etienne
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Sempala, Sylvester D.K.
Uganda, Entebbe
Uganda Virus Research Institute
Wasi, Chantapong
Thailand, Bangkok
Faculty of Medicine Siriraj Hospital, Mahidol University
Osmanov, Saladin K.
Switzerland, Geneva
Organisation Mondiale de la Santé
Fenyö, Éva Mária
Sweden, Solna
Department of Microbiology, Tumor and Cell Biology
Statistics
Citations: 54
Authors: 14
Affiliations: 9
Identifiers
Doi:
10.1089/aid.1994.10.1401
ISSN:
08892229
e-ISSN:
19318405
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Rwanda
Uganda