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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Adaptive Immune Responses in Humans during Nipah Virus Acute and Convalescent Phases of Infection
Clinical Infectious Diseases, Volume 69, No. 10, Year 2019
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Description
Background: Nipah virus (NiV) is 1 of 10 potential causes of imminent public health emergencies of international concern. We investigated the NiV outbreak that occurred in May 2018 in Kerala, India. Here we describe the longitudinal characteristics of cell-mediated and humoral immune responses to NiV infection during the acute and convalescent phases in 2 human survivors. Methods: Serial blood samples were obtained from the only 2 survivors of the NiV outbreak in Kerala. We used flow cytometry to determine the absolute T-lymphocyte and B-lymphocyte counts and the phenotypes of both T and B cells. We also detected and quantitated the humoral immune response to NiV by virus-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) enzyme-linked immunosorbent assay. Results: Absolute numbers of T lymphocytes remained within normal limits throughout the period of illness studied in both survivors. However, a marked elevation of activated CD8 T cells was observed in both cases. More than 30% of total CD8 T cells expressed Ki67, indicating active proliferation. Proliferating (Ki-67+) CD8 T cells expressed high levels of granzyme B and PD-1, consistent with the profile of acute effector cells. Total B-lymphocyte, activated B-cell, and plasmablast counts were also elevated in NiV survivors. These individuals developed detectable NiV-specific IgM and IgG antibodies within a week of disease onset. Clearance of NiV RNA from blood preceded the appearance of virus-specific IgG and coincided with the peak of activated CD8 T cells. Conclusions: We describe for the first time longitudinal kinetic data on the activation status of human B- and T-cell populations during acute NiV infection. While marked CD8 T-cell activation was observed with effector characteristics, activated CD4 T cells were less prominent. © 2019 The Author(s) . Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Arunkumar, Govindakarnavar
India, Manipal
Manipal Academy of Higher Education
McElroy, Anita K.
United States, Atlanta
Centers for Disease Control and Prevention
United States, Pittsburgh
University of Pittsburgh
Klena, John David
United States, Atlanta
Centers for Disease Control and Prevention
Spiropoulou, Christina F.
United States, Atlanta
Centers for Disease Control and Prevention
Laserson, Kayla F.
United States, Atlanta
Centers for Disease Control and Prevention
Nichol, Stuart T.
United States, Atlanta
Centers for Disease Control and Prevention
Statistics
Citations: 22
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1093/cid/ciz010
ISSN:
10584838
Study Design
Cohort Study