Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells
Toxicology in Vitro, Volume 26, No. 2, Year 2012
Notification
URL copied to clipboard!
Description
Titanium dioxide nanoparticles (TiO 2-NPs) induced cytotoxicity and DNA damage have been investigated using human amnion epithelial (WISH) cells, as an in vitro model for nanotoxicity assessment. Crystalline, polyhedral rutile TiO 2-NPs were synthesized and characterized using X-ray diffraction (XRD), UV-Visible spectroscopy, Fourier transform infra red (FTIR) spectroscopy, and transmission electron microscopic (TEM) analyses. The neutral red uptake (NRU) and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assays revealed the concentration dependent cytotoxic effects of TiO 2-NPs (30.6nm) in concentration range of 0.625-10μg/ml. Cells exposed to TiO 2-NPs (10μg/ml) exhibited significant reduction (46.3% and 34.6%; p<0.05) in catalase activity and glutathione (GSH) level, respectively. Treated cells showed 1.87-fold increase in intracellular reactive oxygen species (ROS) generation and 7.3% (p<0.01) increase in G 2/M cell cycle arrest, as compared to the untreated control. TiO 2-NPs treated cells also demonstrated the formation of DNA double strand breaks with 14.6-fold (p<0.05) increase in Olive tail moment (OTM) value at 20μg/ml concentration, vis-à-vis untreated control, under neutral comet assay conditions. Thus, the reduction in cell viability, morphological alterations, compromised antioxidant system, intracellular ROS production, and significant DNA damage in TiO 2-NPs exposed cells signify the potential of these NPs to induce cyto- and genotoxicity in cultured WISH cells. © 2011 Elsevier Ltd.
Authors & Co-Authors
Saquib, Quaiser
Saudi Arabia, Riyadh
College of Sciences
Al-Khedhairy, Abdulaziz Ali A.
Saudi Arabia, Riyadh
College of Sciences
A.Siddiqui, Maqsood Ahmed
Saudi Arabia, Riyadh
College of Sciences
Abou-Tarboush, Faisal Mohamed
Saudi Arabia, Riyadh
College of Sciences
Azam, Ameer T.M.Zafrul
Saudi Arabia, Jeddah
King Abdulaziz University
Musarrat, Javed
Saudi Arabia, Riyadh
College of Sciences
India, Aligarh
Aligarh Muslim University
Statistics
Citations: 239
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.tiv.2011.12.011
ISSN:
08872333
e-ISSN:
18793177
Research Areas
Genetics And Genomics