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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Previously transmitted HIV-1 strains are preferentially selected during subsequent sexual transmissions
Journal of Infectious Diseases, Volume 206, No. 9, Year 2012
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Description
Background. A genetic bottleneck is known to exist for human immunodeficiency virus (HIV) at the point of sexual transmission. However, the nature of this bottleneck and its effect on viral diversity over time is unclear. Methods. Interhost and intrahost HIV diversity was analyzed in a stable population in Rakai, Uganda, from 1994 to 2002. HIV-1 envelope sequences from both individuals in initially HIV-discordant relationships in which transmission occurred later were examined using Sanger sequencing of bulk polymerase chain reaction (PCR) products (for 22 couples), clonal analysis (for 3), and next-generation deep sequencing (for 9). Results. Intrahost viral diversity was significantly higher than changes in interhost diversity (P <. 01). The majority of HIV-1-discordant couples examined via bulk PCR (16 of 22 couples), clonal analysis (3 of 3), and next-generation deep sequencing (6 of 9) demonstrated that the viral populations present in the newly infected recipient were more closely related to the donor partner's HIV-1 variants found earlier during infection as compared to those circulating near the estimated time of transmission (P =. 03). Conclusions. These findings suggest that sexual transmission constrains viral diversity at the population level, partially because of the preferential transmission of ancestral as opposed to contemporary strains circulating in the transmitting partner. Future successful vaccine strategies may need to target these transmitted ancestral strains. © 2012 The Author. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3466994/bin/supp_206_9_1433__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3466994/bin/supp_jis503_jis503supp.docx
Authors & Co-Authors
Redd, Andrew D.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Collinson-Streng, Aleisha N.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Chatziandreou, Nikolaos
United States, Boston
Brigham and Women's Hospital
Mullis, Caroline E.
United States, Baltimore
Johns Hopkins University
Laeyendecker, Oliver B.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
United States, Baltimore
Johns Hopkins University
Martens, Craig A.
United States, Bethesda
National Institutes of Health Nih
Ricklefs, Stacy M.
United States, Bethesda
National Institutes of Health Nih
Kiwanuka, Noah
Uganda, Kampala
Makerere University
Nyein, Phyu Hninn
United States, Boston
Brigham and Women's Hospital
Lutalo, Tom
Uganda, Kampala
Makerere University
Grabowski, Mary Kate
United States, Baltimore
Johns Hopkins Bloomberg School of Public Health
Kong, Xiangrong
United States, Baltimore
Johns Hopkins Bloomberg School of Public Health
Manucci, Jordyn L.
United States, Baltimore
Johns Hopkins University
Sewankambo, Nelson K.
Uganda, Kampala
Makerere University
Wawer, Maria J.
United States, Baltimore
Johns Hopkins Bloomberg School of Public Health
Gray, Ronald H.
United States, Baltimore
Johns Hopkins Bloomberg School of Public Health
Porcella, Stephen F.
United States, Bethesda
National Institutes of Health Nih
Fauci, Anthony S.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Sagar, Manish
United States, Boston
Brigham and Women's Hospital
Serwadda, David Musoke
Uganda, Kampala
Makerere University
Quinn, Thomas Charles
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
United States, Baltimore
Johns Hopkins University
Statistics
Citations: 76
Authors: 21
Affiliations: 6
Identifiers
Doi:
10.1093/infdis/jis503
ISSN:
00221899
Research Areas
Genetics And Genomics
Infectious Diseases
Sexual And Reproductive Health
Study Design
Cross Sectional Study
Study Locations
Uganda