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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Exploiting human CD34
+
stem cell-conditioned medium for tissue repair
Molecular Therapy, Volume 22, No. 1, Year 2014
Notification
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Description
Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34 + cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34 + cells. Thirty-two abundantly secreted factors have been identified, all of which are associated with cell proliferation, survival, tissue repair, and wound healing. The cultured CD34 + cells expressed known stem cell genes such as Nanog, Oct4, Sox2, c-kit, and HoxB4. The conditioned medium containing the secreted factors prevented cell death in liver cells exposed to liver toxin in vitro via inhibition of the caspase-3 signaling pathway. More importantly, in vivo studies using animal models of liver damage demonstrated that injection of the conditioned medium could repair damaged liver tissue (significant reduction in the necroinflammatory activity), as well as enable the animals to survive. Thus, we demonstrate that medium conditioned by human CD34 + cells has the potential for therapeutic repair of damaged tissue in vivo. © The American Society of Gene & Cell Therapy.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3978788/bin/mt2013194x1.pdf
Authors & Co-Authors
Mintz, Paul J.
United Kingdom, London
Imperial College London
Qatar, Doha
Qatar Biomedical Research Institute
Huang, Kai Wen
Taiwan, Taipei
National Taiwan University Hospital
Taiwan, Taipei
National Taiwan University
Reebye, Vikash
United Kingdom, London
Imperial College London
Nteliopoulos, Georgios
United Kingdom, London
Imperial College London
Lai, Hong Shiee
Taiwan, Taipei
National Taiwan University Hospital
Taiwan, Taipei
National Taiwan University
Sætrom, Pål
Norway, Trondheim
Norges Teknisk-naturvitenskapelige Universitet
Kasahara, Noriyuki A.
United States, Los Angeles
David Geffen School of Medicine at Ucla
Jensen, Steen Lindkaer
United Kingdom, London
Imperial College London
Pai, Madhava
United Kingdom, London
Imperial College London
Gordon, Myrtle Ya
United Kingdom, London
Imperial College London
Marley, Stephen B.
United Kingdom, London
Imperial College London
Behan, Rosemary
United Kingdom, London
Imperial College London
Spalding, Duncan R.C.
United Kingdom, London
Imperial College London
Haoudi, Abdelali
Qatar, Doha
Qatar Biomedical Research Institute
Emara, Mohamed M.
Qatar, Doha
Qatar Biomedical Research Institute
Egypt, Giza
Faculty of Veterinary Medicine
Nicholls, Joanna P.
United Kingdom, London
Imperial College London
Rossi, John J.
United States, Duarte
City of Hope National Med Center
Habib, Nagy A.
United Kingdom, London
Imperial College London
Statistics
Citations: 18
Authors: 18
Affiliations: 8
Identifiers
Doi:
10.1038/mt.2013.194
ISSN:
15250016
e-ISSN:
15250024
Research Areas
Cancer
Genetics And Genomics