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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients
Fertility and Sterility, Volume 83, No. 3, Year 2005
Notification
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Description
Objective: To investigate whether there is an impact of different sources of reactive oxygen species (ROS) on sperm functions. Design: Prospective study. Setting: Patients at the Center for Dermatology and Andrology, Giessen, Germany. Patient(s): Semen collected from 63 randomly collected patients attending the IVF unit of the University of Giessen, Germany. Intervention(s): Only patients with nonleukocytospermia were included in this study. Main Outcome Measure(s): Sperm count and motility before and after sperm separation by swim-up, morphology, DNA fragmentation, and extrinsic (by leukocytes) and intrinsic ROS production (by spermatozoa) were evaluated. Result(s): Leukocytes correlated significantly with extrinsic ROS production (r = 0.576), but markedly less with intrinsic ROS production (r = 0.296). Sperm count, morphology, and motility in the ejaculate were markedly more affected by extrinsic than by intrinsic ROS. The DNA fragmentation was strongly positively correlated with intrinsic ROS production, whereas this correlation was weaker for extrinsic ROS production. No correlation was found between DNA fragmentation and the number of leukocytes, whereas the correlations with motility in the ejaculate and the motile sperm count after swim-up were highly significant. Moreover, significant differences were observed for extrinsic and intrinsic ROS production between groups of patients having a high (<1 × 106/mL) and a low number (<1 × 106/mL) of leukocytes in the ejaculate. Conclusion(s): The origin of ROS seems to have an influence on the site of the damage. Because leukocyte counts <1 × 106/mL caused a significant decrease of motility and DNA integrity, the threshold given by the World Health Organization (WHO) should be re-evaluated. © 2005 by American Society for Reproductive Medicine.
Authors & Co-Authors
Henkel, Ralf Reinhold
Germany, Giessen
Justus-liebig-universität Gießen
Germany, Jena
Friedrich-schiller-universität Jena
Kierspel, Eva
Germany, Giessen
Justus-liebig-universität Gießen
Stalf, Thomas
Germany, Giessen
Justus-liebig-universität Gießen
Mehnert, Claas
Germany, Giessen
Justus-liebig-universität Gießen
Menkveld, Roelof
South Africa, Tygerberg
Tygerberg Hospital
Tinneberg, Hans Rudolf
Germany, Giessen
Justus-liebig-universität Gießen
Schill, Wolf Bernhard
Germany, Giessen
Justus-liebig-universität Gießen
Kruger, Theunis Frans
South Africa, Tygerberg
Tygerberg Hospital
Statistics
Citations: 303
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/j.fertnstert.2004.11.022
ISSN:
00150282
Research Areas
Genetics And Genomics
Health System And Policy
Sexual And Reproductive Health
Study Design
Randomised Control Trial
Cohort Study