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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Development of a fluorescent microbead-based immunoassay for the detection of hepatitis E virus IgG antibodies in pigs and comparison to an enzyme-linked immunoassay
Journal of Virological Methods, Volume 193, No. 2, Year 2013
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Description
Swine hepatitis E virus (HEV) is a zoonotic virus and pigs are considered as an important reservoir. Swine HEV infection is widespread and most pig herds are infected. Humans can be infected with swine HEV via consumption of undercooked pork or through direct contact with infected pigs. To minimize the risk of zoonotic transmission, sensitive tools to assess the HEV infection status of pigs and pork products are needed. The objective of this study was to develop a fluorescent microbead-based immunoassay (FMIA) for the detection of IgG antibodies against swine HEV and compare it to an in-house enzyme-linked immunoassay (ELISA). Three sets of samples were utilized: (A) samples from pigs infected experimentally with different strains of HEV (positive controls, n= 72), (B) samples from known HEV-negative pigs (negative controls, n= 62) and (C) samples from pigs of unknown HEV infection status (n= 182). All samples were tested by both ELISA and FMIA. The results on the experimental samples with known HEV exposure indicate that both assays have a specificity of 100% while the sensitivity ranges from 84.6% (ELISA) to 92.3% (FMIA). The overall prevalence of HEV IgG antibodies in field samples from pigs with unknown HEV exposure was 21.9% (40/182) for the ELISA and 21.4% (39/182) for the FMIA. The two assays had an almost perfect overall agreement (Kappa= 0.92). © 2013 Elsevier B.V.
Authors & Co-Authors
Owolodun, Olajide Adewale
United States, Ames
Iowa State University
Nigeria, Vom
National Veterinary Research Institute, Vom
Giménez-Lirola, Luis G.
United States, Ames
Iowa State University
Gerber, P. F.
United States, Ames
Iowa State University
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Sanford, Brenton J.
United States, Blacksburg
Center for Molecular Medicine and Infectious Diseases
Feagins, Alicia R.
United States, Blacksburg
Center for Molecular Medicine and Infectious Diseases
Meng, Xiang Jin
United States, Blacksburg
Center for Molecular Medicine and Infectious Diseases
Halbur, Patrick G.
United States, Ames
Iowa State University
Opriessnig, Tanja
United States, Ames
Iowa State University
Statistics
Citations: 8
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1016/j.jviromet.2013.06.010
ISSN:
01660934
e-ISSN:
18790984
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study