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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
How well do ITS rDNA sequences differentiate species of true morels (Morchella)?
Mycologia, Volume 104, No. 6, Year 2012
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Description
Arguably more mycophiles hunt true morels (Morchella) during their brief fruiting season each spring in the northern hemisphere than any other wild edible fungus. Concerns about overharvesting by individual collectors and commercial enterprises make it essential that science-based management practices and conservation policies are developed to ensure the sustainability of commercial harvests and to protect and preserve morel species diversity. Therefore, the primary objectives of the present study were to: (i) investigate the utility of the ITS rDNA locus for identifying Morchella species, using phylogenetic species previously inferred from multilocus DNA sequence data as a reference; and (ii) clarify insufficiently identified sequences and determine whether the named sequences in GenBank were identified correctly. To this end, we generated 553 Morchella ITS rDNA sequences and downloaded 312 additional ones generated by other researchers from GenBank using emerencia and analyzed them phylogenetically. Three major findings emerged: (i) ITS rDNA sequences were useful in identifying 48/62 (77.4%) of the known phylospecies; however, they failed to identify 12 of the 22 species within the species-rich Elata Subclade and two closely related species in the Esculenta Clade; (ii) at least 66% of the named Morchella sequences in GenBank are misidentified; and (iii) ITS rDNA sequences of up to six putatively novel Morchella species were represented in GenBank. Recognizing the need for a dedicated Webaccessible reference database to facilitate the rapid identification of known and novel species, we constructed Morchella MLST (http://www.cbs.knaw. nl/morchella/), which can be queried with ITS rDNA sequences and those of the four other genes used in our prior multilocus molecular systematic studies of this charismatic genus. © 2012 by The Mycological Society of America.
Authors & Co-Authors
Zhao, Qi
China, Beijing
Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Yang, Zhuliang
China, Beijing
Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Hansen, Karen
Sweden, Stockholm
Naturhistoriska Riksmuseet
Moncalvo, Jean Marc
Canada, Toronto
University of Toronto
Canada, Toronto
Royal Ontario Museum
Douhan, Greg W.
United States, Riverside
University of California, Riverside
Robert, Vincent A.R.G.
Netherlands, Utrecht
Westerdijk Fungal Biodiversity Institute - Knaw
Crous, Pedro Willem
Netherlands, Utrecht
Westerdijk Fungal Biodiversity Institute - Knaw
Rehner, Stephen A.
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
Rooney, A. P.
United States, Washington, D.c.
Usda Agricultural Research Service
O\'Donnell, Kerry L.
United States, Washington, D.c.
Usda Agricultural Research Service
Statistics
Citations: 56
Authors: 10
Affiliations: 10
Identifiers
Doi:
10.3852/12-056
ISSN:
15572536
Research Areas
Cancer
Genetics And Genomics