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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Aberrant methylation of RARβ
2
and APC genes in voided urine as molecular markers for early detection of bilharzial and nonbilharzial bladder cancer
Cancer Epidemiology Biomarkers and Prevention, Volume 20, No. 8, Year 2011
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Description
Background: Bladder cancer cells illustrate major disruptions in their DNA methylation patterns as compared with normal ones. Authors aimed to identify epigenetic molecular markers in urine for early detection of bladder cancer. Materials and Methods: We retrospectively analyzed the methylation status of RARβ2 and APC genes in urine samples from 210 bladder cancer patients, 61 patients with benign urological diseases, and 49 healthy volunteers by using methylation-specific PCR. Results: Methylated RARβ2 and APC were significantly higher in bladder cancer patients (62.8%, 59.5%) than benign (16.4%, 5%) but not detected in healthy volunteers (0%) at (P < 0.0001). Both methylated genes showed no significant difference among clinicopathologic factors; however, they were detected in all grades and stages. Among the 128 patients with bilharzial bladder cancer, 94 (73.4%) showed methylated RARβ2 and 86 (67.2%) showed methylated APC. Homoplasmic methylation pattern of both genes were only detected in bilharzial bladder cancer cases. Both sensitivities and specificities of the methylated genes for bladder cancer detection were superior to urine cytology and when altogether combined, the sensitivities improved to (91.8%), (93.5%), (91.9%), and (80.9%) in detection of: bladder cancer, non-muscle invasive bladder cancer, low-grade tumors, and bilharzial associated bladder cancer, respectively. Conclusion: Thus, methylated RARβ2 and APC genes might be valuable urinary molecular markers for early detection of bilharzial and nonbilharzial bladder cancer. ©2011 AACR.
Authors & Co-Authors
Eissa, S.
Egypt, Cairo
Faculty of Medicine
Swellam, Menha M.
Egypt, Giza
Cairo University
El-Khouly, Inas M.
Egypt, Cairo
Faculty of Medicine
Kassim, Samar Kamal
Egypt, Cairo
Faculty of Medicine
Shehata, Hanan Hussein
Egypt, Cairo
Faculty of Medicine
Mansour, Amal A.
Egypt, Cairo
Faculty of Medicine
Esmat, Mohamed
Egypt, Cairo
Faculty of Medicine - Ain Shams University
Nossier, Ahmed Ibrahim
Egypt, 6th October
Misr University for Science and Technology
Hamdy, Mohamed Ahmed
Egypt, Cairo
Faculty of Pharmacy
Awad, Nahla M.
Egypt, Cairo
Faculty of Medicine - Ain Shams University
El-Ahmady, Omar E.
Egypt, Cairo
Faculty of Pharmacy - Ain Shams University
Statistics
Citations: 44
Authors: 11
Affiliations: 6
Identifiers
Doi:
10.1158/1055-9965.EPI-11-0237
ISSN:
10559965
Research Areas
Cancer
Genetics And Genomics