Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Diagnostic gene expression biomarkers of coral thermal stress
Molecular Ecology Resources, Volume 14, No. 4, Year 2014
Notification
URL copied to clipboard!
Description
Gene expression biomarkers can enable rapid assessment of physiological conditions in situ, providing a valuable tool for reef managers interested in linking organism physiology with large-scale climatic conditions. Here, we assessed the ability of quantitative PCR (qPCR)-based gene expression biomarkers to evaluate (i) the immediate cellular stress response (CSR) of Porites astreoides to incremental thermal stress and (ii) the magnitude of CSR and cellular homeostasis response (CHR) during a natural bleaching event. Expression levels largely scaled with treatment temperature, with the strongest responses occurring in heat-shock proteins. This is the first demonstration of a 'tiered' CSR in a coral, where the magnitude of expression change is proportional to stress intensity. Analysis of a natural bleaching event revealed no signature of an acute CSR in normal or bleached corals, indicating that the bleaching stressor(s) had abated by the day of sampling. Another long-term stress CHR-based indicator assay was significantly elevated in bleached corals, although assay values overall were low, suggesting good prospects for recovery. This study represents the first step in linking variation in gene expression biomarkers to stress tolerance and bleaching thresholds in situ by quantifying the severity of ongoing thermal stress and its accumulated long-term impacts. © 2013 John Wiley & Sons Ltd.
Authors & Co-Authors
Kenkel, C. D.
United States, Austin
The University of Texas at Austin
Sheridan, Christopher
Belgium, Mons
Université de Mons
Leal, Miguel C.
Portugal, Aveiro
Universidade de Aveiro
United States, Savannah
Skidaway Institute of Oceanography
Bhagooli, Ranjeet
Mauritius, Reduit
University of Mauritius
Castillo, K. D.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Kurata, N.
United States, Jacksonville
University of North Florida
Mcginty, E.
United States, Arlington
The University of Texas at Arlington
Goulet, T. L.
United States, University
University of Mississippi
Matz, M. V.
United States, Austin
The University of Texas at Austin
Statistics
Citations: 57
Authors: 9
Affiliations: 9
Identifiers
Doi:
10.1111/1755-0998.12218
ISSN:
1755098X
e-ISSN:
17550998
Research Areas
Environmental
Genetics And Genomics
Study Approach
Quantitative