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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mapping a Quantitative Trait Locus (QTL) conferring pyrethroid resistance in the African malaria vector Anopheles funestus
BMC Genomics, Volume 8, Article 34, Year 2007
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Description
Background: Pyrethroid resistance in Anopheles funestus populations has led to an increase in malaria transmission in southern Africa. Resistance has been attributed to elevated activities of cytochrome P450s but the molecular basis underlying this metabolic resistance is unknown. Microsatellite and SNP markers were used to construct a linkage map and to detect a quantitative trait locus (QTL) associated with pyrethroid resistance in the FUMOZ-R strain of An. funestus from Mozambique. Results: By genotyping 349 F2 individuals from II independent families, a single major QTL, rp1, at the telomeric end of chromosome 2R was identified. The rp1 QTL appears to present a major effect since it accounts for more than 60% of the variance in susceptibility to permethrin. This QTL has a strong additive genetic effect with respect to susceptibility. Candidate genes associated with pyrethroid resistance in other species were physically mapped to An. funestus polytene chromosomes. This showed that rp1 is genetically linked to a cluster of CYP6 cytochrome P450 genes located on division 9 of chromosome 2R and confirmed earlier reports that pyrethroid resistance in this strain is not associated with target site mutations (knockdown resistance). Conclusion: We hypothesize that one or more of these CYP6 P450s clustered on chromosome 2R confers pyrethroid resistance in the FUMOZ-R strain of An. funestus. © 2007 Wondji et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Wondji, Charles Sinclair
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Morgan, John C.
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Coetzee, Maureen
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
University of the Witwatersrand
Hunt, Richard H.
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
University of the Witwatersrand
Steen, Keith
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Black IV, William C.
United States, Fort Collins
Colorado State University
Hemingway, Janet
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Ranson, Hilary A.
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Statistics
Citations: 88
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1186/1471-2164-8-34
e-ISSN:
14712164
Research Areas
Genetics And Genomics
Infectious Diseases
Noncommunicable Diseases
Study Approach
Quantitative
Study Locations
Mozambique