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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Differential cellular gene expression in ganglioglioma
Epilepsia, Volume 48, No. 1, Year 2007
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Description
Purpose: Gangliogliomas (GGs) are neuronal-glial tumors highly associated with epilepsy. We hypothesized that the expression of select gene families including neurotransmitter receptor subunits and growth factors would be distinct in neurons and astrocytes within GG compared with adjacent cortex and that these changes would yield insights into seizure onset and lesion formation. Methods: Candidate gene expression was defined in single immunohistochemically labeled neurons and astrocytes microdissected from GG specimens compared with neurons and astrocytes microdissected from morphologically intact cortex adjacent to the GG or normal control cortex. Results: Differential expression of 16 genes including glutamate transporter (EAAC1) and receptor (NMDA2C, mGluR5), growth factor (hepatocyte growth factor), and receptor (platelet derived growth factor receptor β, fibroblast growth factor receptor 3) mRNAs was detected in GG neurons compared with control neurons. In astrocytes, altered expression of p75NGF, mGluR3, TGFβ3 and Glt-1 mRNAs was detected. Nestin mRNA, a gene that exhibits enhanced expression in balloon cell cortical dysplasia, was increased in GG neurons. Because of the morphological similarities between GG and cortical dysplasia, we show that there is activation of the mTOR cascade in GG as evidenced by enhanced expression of phospho-p70S6kinase and phosphoribosomal S6 proteins. Conclusion: We find differential candidate gene expression in neurons and astrocytes in GG compared with adjacent cortex and show that there is activation of the mTOR pathway. These changes highlight pathways that may be pivotal for epileptogenesis and lesion growth. © 2007 International League Against Epilepsy.
Authors & Co-Authors
Samadani, Uzma
United States, Philadelphia
Hospital of the University of Pennsylvania
Judkins, Alexander R.
United States, Philadelphia
University of Pennsylvania Perelman School of Medicine
Akpalu, Albert K.
Ghana, Accra
University of Ghana
Aronica, Eleonora
Netherlands, Amsterdam
Universiteit Van Amsterdam
Crino, Peter B.
United States, Philadelphia
Hospital of the University of Pennsylvania
Statistics
Citations: 5
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/j.1528-1167.2007.00925.x
e-ISSN:
15281167
Research Areas
Genetics And Genomics