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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Analysis of circulating tumour cells in early-stage uveal melanoma: Evaluation of tumour marker expression to increase capture
Cancers, Volume 13, No. 23, Article 5990, Year 2021
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Description
(1) Background: The stratification of uveal melanoma (UM) patients into prognostic groups is critical for patient management and for directing patients towards clinical trials. Current classification is based on clinicopathological and molecular features of the tumour. Analysis of circulating tumour cells (CTCs) has been proposed as a tool to avoid invasive biopsy of the primary tumour. However, the clinical utility of such liquid biopsy depends on the detection rate of CTCs. (2) Methods: The expression of melanoma, melanocyte, and stem cell markers was tested in a primary tissue microarray (TMA) and UM cell lines. Markers found to be highly expressed in primary UM were used to either immunomagnetically isolate or immunostain UM CTCs prior to treatment of the primary lesion. (3) Results: TMA and cell lines had heterogeneous expression of common melanoma, melanocyte, and stem cell markers. A multi-marker panel of immunomagnetic beads enabled isolation of CTCs in 37/43 (86%) patients with UM. Detection of three or more CTCs using the multi-marker panel, but not MCSP alone, was a significant predictor of shorter progression free (p = 0.040) and overall (p = 0.022) survival. (4) Conclusions: The multi-marker immunomagnetic isolation protocol enabled the detection of CTCs in most primary UM patients. Overall, our results suggest that a multi-marker approach could be a powerful tool for CTC separation for non-invasive prognostication of UM. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Authors & Co-Authors
Chen, Fred Kuanfu
Australia, Perth
The University of Western Australia
Australia, Perth
Royal Perth Hospital
Erber, Wendy Naomi
Australia, Perth
The University of Western Australia
Ziman, Mel
Australia, Perth
Edith Cowan University
Australia, Perth
The University of Western Australia
Gray, Elin Solomonovna
Australia, Perth
Edith Cowan University
Statistics
Citations: 3
Authors: 4
Affiliations: 6
Identifiers
Doi:
10.3390/cancers13235990
ISSN:
20726694
Research Areas
Cancer
Health System And Policy