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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Deletion of IL-4Rα on CD4 T cells renders BALB/c mice resistant to Leishmania major infection
PLoS Pathogens, Volume 3, No. 5, Year 2007
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Description
Effector responses induced by polarized CD4+ T helper 2 (Th2) cells drive nonhealing responses in BALB/c mice infected with Leishmania major. Th2 cytokines IL-4 and IL-13 are known susceptibility factors for L. major infection in BALB/c mice and induce their biological functions through a common receptor, the IL-4 receptor α chain (IL-4Rα). IL-4Rα-deficient BALB/c mice, however, remain susceptible to L. major infection, indicating that IL-4/IL-13 may induce protective responses. Therefore, the roles of polarized Th2 CD4+ T cells and IL-4/IL-13 responsiveness of non-CD4+ T cells in inducing nonhealer or healer responses have yet to be elucidated. CD4+ T cell-specific IL-4Rα (LckcreIL- 4Rα-/lox) deficient BALB/c mice were generated and characterized to elucidate the importance of IL-4Rα signaling during cutaneous leishmaniasis in the absence of IL-4-responsive CD4+ T cells. Efficient deletion was confirmed by loss of IL-4Rα expression on CD4+ T cells and impaired IL-4-induced CD4+ T cell proliferation and Th2 differentiation. CD8+, γδ +, and NK-T cells expressed residual IL-4Rα, and representative non-T cell populations maintained IL-4/IL-13 responsiveness. In contrast to IL-4Rα-/lox BALB/c mice, which developed ulcerating lesions following infection with L. major, LckcreIL-4Rα-/lox mice were resistant and showed protection to rechallenge, similar to healer C57BL/6 mice. Resistance to L. major in LckcreIL-4Rα -/lox mice correlated with reduced numbers of IL-10-secreting cells and early IL-12p35 mRNA induction, leading to increased delayed type hypersensitivity responses, interferon-γ production, and elevated ratios of inducible nitric oxide synthase mRNA/parasite, similar to C57BL/6 mice. These data demonstrate that abrogation of IL-4 signaling in CD4+ T cells is required to transform nonhealer BALB/c mice to a healer phenotype. Furthermore, a beneficial role for IL-4Rα signaling in L. major infection is revealed in which IL-4/IL-13-responsive non-CD4+ T cells induce protective responses. © 2007 Radwanska et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1867380/bin/ppat.0030068.sg001.pdf
Authors & Co-Authors
Radwanska, Magdalena
South Africa, Cape Town
University of Cape Town
Cutler, Antony J.
South Africa, Cape Town
University of Cape Town
Hoving, Jennifer Claire
South Africa, Cape Town
University of Cape Town
Magez, Stefan
South Africa, Cape Town
University of Cape Town
Belgium, Brussels
Vrije Universiteit Brussel
Hólscher, Christoph
South Africa, Cape Town
University of Cape Town
Bohms, Andreas
South Africa, Cape Town
University of Cape Town
Arendse, Berenice
South Africa, Cape Town
University of Cape Town
Kirsch, Richard H.
South Africa, Cape Town
University of Cape Town
Hünig, Thomas R.
Germany, Wurzburg
Julius-maximilians-universität Würzburg
Alexander, James B.
United Kingdom, Glasgow
University of Strathclyde
Kaye, Paul Martin
United Kingdom, York
University of York
Brombacher, Frank
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 65
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1371/journal.ppat.0030068
ISSN:
15537366
e-ISSN:
15537374