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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Population structure of Pseudomonas aeruginosa from five Mediterranean countries: Evidence for frequent recombination and epidemic occurrence of CC235
PLoS ONE, Volume 6, No. 10, Article e25617, Year 2011
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Description
Several studies in recent years have provided evidence that Pseudomonas aeruginosa has a non-clonal population structure punctuated by highly successful epidemic clones or clonal complexes. The role of recombination in the diversification of P. aeruginosa clones has been suggested, but not yet demonstrated using multi-locus sequence typing (MLST). Isolates of P. aeruginosa from five Mediterranean countries (n = 141) were subjected to pulsed-field gel electrophoresis (PFGE), serotyping and PCR targeting the virulence genes exoS and exoU. The occurrence of multi-resistance (≥3 antipseudomonal drugs) was analyzed with disk diffusion according to EUCAST. MLST was performed on a subset of strains (n = 110) most of them had a distinct PFGE variant. MLST data were analyzed with Bionumerics 6.0, using minimal spanning tree (MST) as well as eBURST. Measurement of clonality was assessed by the standardized index of association (I AS). Evidence of recombination was estimated by ClonalFrame as well as SplitsTree4.0. The MST analysis connected 70 sequence types, among which ST235 was by far the most common. ST235 was very frequently associated with the O11 serotype, and frequently displayed multi-resistance and the virulence genotype exoS -/exoU +. ClonalFrame linked several groups previously identified by eBURST and MST, and provided insight to the evolutionary events occurring in the population; the recombination/mutation ratio was found to be 8.4. A Neighbor-Net analysis based on the concatenated sequences revealed a complex network, providing evidence of frequent recombination. The index of association when all the strains were considered indicated a freely recombining population. P. aeruginosa isolates from the Mediterranean countries display an epidemic population structure, particularly dominated by ST235-O11, which has earlier also been coupled to the spread of ß-lactamases in many countries. © 2011 Maatallah et al.
Authors & Co-Authors
Maãtallah, Makaoui
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Cheriaa, Jihane
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Backhrouf, Amina
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Iversen, Aina
Sweden, Stockholm
Karolinska Universitetssjukhuset
Grundmann, Hajo J.
Netherlands, Groningen
Universitair Medisch Centrum Groningen
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Do, Thuy
United Kingdom, London
King's College London
Lanotte, Philippe
France, Tours
Hopital Bretonneau
France, Tours
Université de Tours
Mastouri, Maha
Tunisia, Monastir
Chu Fattouma-bourguiba
Elghmati, Mohamed Salem
Libya, Tripoli
University of Tripoli
Rojo, Fernando
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Snuossi, Mejdi
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Giske, Christian Georg
Sweden, Stockholm
Karolinska Universitetssjukhuset
Statistics
Citations: 142
Authors: 12
Affiliations: 10
Identifiers
Doi:
10.1371/journal.pone.0025617
e-ISSN:
19326203
Research Areas
Cancer
Genetics And Genomics
Study Design
Cross Sectional Study