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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Evaluation of envelope vaccines derived from the South African subtype C human immunodeficiency virus type 1 TV1 strain
Journal of Virology, Volume 79, No. 21, Year 2005
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Description
Human immunodeficiency virus type 1 (HIV-1) subtype C infections are on the rise in Sub-Saharan Africa and Asia. Therefore, there is a need to develop an HIV vaccine capable of eliciting broadly reactive immune responses against members of this subtype. We show here that modified HIV envelope (env) DNA vaccines derived from the South African subtype C TV1 strain are able to prime for Immoral responses in rabbits and rhesus macaques. Priming rabbits with DNA plasmids encoding V2-deleted TV1 gp140 (gp140TV1ΔV2), followed by boosting with oligomeric protein (o-gp140TV1ΔV2) in MF59 adjuvant, elicited higher titers of env-binding and autologous neutralizing antibodies than priming with DNA vaccines encoding the full-length TV1 env (gp160) or the intact TV1 gp140. Immunization with V2-deleted subtype B SF162 env and V2-deleted TVl env together using a multivalent vaccine approach induced high titers of oligomeric env-binding antibodies and autologous neutralizing antibodies against both the subtypes B and C vaccine strains, HIV-1 SF162 and TV1, respectively. Low-level neutralizing activity against the heterologous South African subtype C TV2 strain, as well as a small subset of viruses in a panel of 13 heterologous primary isolates, was observed in some rabbits immunized with the V2-deleted vaccines. Immunization of rhesus macaques with the V2-deleted TV1 DNA prime/protein boost also elicited high titers of env-binding antibodies and moderate titers of autologous TV1 neutralizing antibodies. The pilot-scale production of the various TV1 DNA vaccine constructs and env proteins described here should provide an initial platform upon which to improve the immunogenicity of these subtype C HIV envelope vaccines. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Lian, Ying
Switzerland, Basel
Novartis International ag
Srivastava, Indresh
Switzerland, Basel
Novartis International ag
Gómez Román, Raúl
Switzerland, Basel
Novartis International ag
Zur Megede, Jan
Switzerland, Basel
Novartis International ag
Sun, Yide
Switzerland, Basel
Novartis International ag
Kan, Elaine
Switzerland, Basel
Novartis International ag
Hilt, Susan
Switzerland, Basel
Novartis International ag
Engelbrecht, Susan
South Africa, Tygerberg
Tygerberg Hospital
Himathongkham, Sunee
United States, Davis
University of California, Davis
United States, Richmond
Food and Drug Laboratory Branch
Luciw, Paul A.
United States, Davis
University of California, Davis
Otten, Gillis
Switzerland, Basel
Novartis International ag
Ulmer, Jeffrey B.
Switzerland, Basel
Novartis International ag
Donnelly, John J.
Switzerland, Basel
Novartis International ag
Rabussay, Dietmar P.
United States, San Diego
Inovio Biomedical Corporation
Montefiori, David Charles
United States, Durham
Duke University Medical Center
van Rensburg, Estrelita Janse
South Africa, Tygerberg
Tygerberg Hospital
South Africa, Pretoria
University of Pretoria
Barnett, Susan W.
Switzerland, Basel
Novartis International ag
Statistics
Citations: 53
Authors: 17
Affiliations: 7
Identifiers
Doi:
10.1128/JVI.79.21.13338-13349.2005
ISSN:
0022538X
Research Areas
Genetics And Genomics
Infectious Diseases
Maternal And Child Health