Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A heterologous prime/boost immunisation strategy protects against virulent E. ruminantium Welgevonden needle challenge but not against tick challenge
Vaccine, Volume 26, No. 34, Year 2008
Notification
URL copied to clipboard!
Description
Heterologous prime/boost immunisation strategies using the Ehrlichia ruminantium 1H12 pCMViUBs_ORFs [Pretorius A, Collins NE, Steyn HC, Van Strijp F, Van Kleef M, Allsopp BA. Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames. Vaccine 2007;25(12):2316-24] were investigated in this study. All the animals immunised twice with a recombinant (r) DNA cocktail of four 1H12 pCMViUBs_ORFs followed by a r1H12 protein and those immunised 3× with 1H12 plasmid rDNA showed 100% protection against a virulent E. ruminantium Welgevonden needle challenge. In addition, 90% of the sheep immunised twice with rDNA and boosted with r1H12 lumpy skin disease virus (LSDV) survived. Only the lymphocytes isolated from the r1H12 protein boost group showed specific proliferation and increased interferon (IFN)-γ expression. In contrast, only 20% protection was obtained in animals immunised with the rDNA prime/r1H12 protein boost when subjected to natural tick challenge in the field. Thus this heterologous prime/boost immunisation strategy had not conferred any significant protection against a field challenge. © 2008 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Pretorius, Alri
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
South Africa, Pretoria
University of Pretoria
van Kleef, Mirinda
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
South Africa, Pretoria
University of Pretoria
Collins, Nicola E.
South Africa, Pretoria
University of Pretoria
Tshikudo, N.
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Louw, Elmarié
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Faber, F. E.
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
van Strijp, M. Fransie
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Allsopp, Basil A.
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 42
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1016/j.vaccine.2008.06.006
ISSN:
0264410X
Research Areas
Genetics And Genomics