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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A TaqMan real-time PCR-based assay for the identification of Fasciola spp.
Veterinary Parasitology, Volume 179, No. 1-3, Year 2011
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Description
Real time quantitative PCR (qPCR) is one of the key technologies of the post-genome era, with clear advantages compared to normal end-point PCR. In this paper, we report the first qPCR-based assay for the identification of Fasciola spp. Based on sequences of the second internal transcribed spacers (ITS-2) of the ribosomal rRNA gene, we used a set of genus-specific primers for Fasciola ITS-2 amplification, and we designed species-specific internal TaqMan probes to identify F. hepatica and F. gigantica, as well as the hybrid 'intermediate' Fasciola. These primers and probes were used for the highly specific, sensitive, and simple identification of Fasciola species collected from different animal host from China, Spain, Niger and Egypt. The novel qPCR-based technique for the identification of Fasciola spp. may provide a useful tool for the epidemiological investigation of Fasciola infection, including their intermediate snail hosts. © 2011 Elsevier B.V.
Authors & Co-Authors
Alasaad, Samer
Spain, Sevilla
Csic - Estación Biologica de Doñana
Soriguer, R. C.
Spain, Sevilla
Csic - Estación Biologica de Doñana
Abu-Madi, Marawan A.
Qatar, Doha
Qatar University
El Behairy, Ahmed M.
Egypt, Giza
Faculty of Veterinary Medicine
Jowers, M. J.
Spain, Sevilla
Csic - Estación Biologica de Doñana
Baños, Pablo Díez
Spain, Santiago de Compostela
Universidad de Santiago de Compostela
Píriz, Ana
Spain, Sevilla
Csic - Estación Biologica de Doñana
Fickel, Jörns
Germany, Berlin
Leibniz-institut Für Zoo- Und Wildtierforschung
Zhu, Xingquan X.
China, Beijing
Chinese Academy of Agricultural Sciences
Statistics
Citations: 46
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1016/j.vetpar.2011.01.059
ISSN:
03044017
Research Areas
Genetics And Genomics
Study Approach
Quantitative
Study Locations
Egypt
Niger