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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
DNA barcoding the floras of biodiversity hotspots
Proceedings of the National Academy of Sciences of the United States of America, Volume 105, No. 8, Year 2008
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Description
DNA barcoding is a technique in which species identification is performed by using DNA sequences from a small fragment of the genome, with the aim of contributing to a wide range of ecological and conservation studies in which traditional taxonomic identification is not practical. DNA barcoding is well established in animals, but there is not yet any universally accepted barcode for plants. Here, we undertook intensive field collections in two biodiversity hotspots (Mesoamerica and southern Africa). Using >1,600 samples, we compared eight potential barcodes. Going beyond previous plant studies, we assessed to what extent a "DNA barcoding gap" is present between intra- and interspecific variations, using multiple accessions per species. Given its adequate rate of variation, easy amplification, and alignment, we identified a portion of the plastid matK gene as a universal DNA barcode for flowering plants. Critically, we further demonstrate the applicability of DNA barcoding for biodiversity inventories. In addition, analyzing > 1,000 species of Mesoamerican orchids, DNA barcoding with matK alone reveals cryptic species and proves useful in identifying species listed in Convention on International Trade of Endangered Species (CITES) appendixes. © 2008 by The National Academy of Sciences of the USA.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_1.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_2.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_3.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_09936Table6.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_09936Table7.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_09936Table8.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_09936Table9.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2268561/bin/pnas_0709936105_09936Table10.xls
Authors & Co-Authors
Lahaye, Renaud
South Africa, Johannesburg
University of Johannesburg
van der Bank, Michelle
South Africa, Johannesburg
University of Johannesburg
Bogarin, Diego
Costa Rica, San Jose
Universidad de Costa Rica
Warner, Jorge
Costa Rica, San Jose
Universidad de Costa Rica
Pupulin, Franco
Costa Rica, San Jose
Universidad de Costa Rica
Gigot, Guillaume
United Kingdom, Richmond
Royal Botanic Gardens, Kew
Maurin, Olivier
South Africa, Johannesburg
University of Johannesburg
Duthoit, Sylvie
South Africa, Johannesburg
University of Johannesburg
Barraclough, Timothy Giles
United Kingdom, London
Imperial College London
Savolainen, Vincent
United Kingdom, Richmond
Royal Botanic Gardens, Kew
United Kingdom, London
Imperial College London
Statistics
Citations: 865
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1073/pnas.0709936105
ISSN:
00278424
Research Areas
Genetics And Genomics