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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
TLTF in cerebrospinal fluid for detection and staging of T. b. gambiense infection
PLoS ONE, Volume 8, No. 11, Article e79281, Year 2013
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Description
Background: Trypanosome-derived lymphocyte triggering factor (TLTF) is a molecule released by African trypanosomes that interacts with the host immune system, resulting in increased levels of IFN-γ production. Methodology/Principal findings: TLTF and anti-TLTF antibodies were assessed in sera and cerebrospinal fluid (CSF) from patients infected with Trypanosoma brucei gambiense (T. b. gambiense) in an attempt to identify alternative markers for diagnosis and stage determination of human African trypanosomiasis or sleeping sickness. Seventy-four serum and sixtyone CSF samples from patients with parasitologically confirmed infection and known disease stage along with 13 sera and CSF from uninfected controls were tested. In serum the levels of anti-TLTF antibodies were unrelated to the disease stage. In contrast, levels of anti-TLTF antibodies in CSF were higher in intermediate/late stages than in early stage disease patients. Specificity of the detected antibodies was assessed by inhibition of TLTF bioactivity as represented by its ability to induce IFN-γ production. Additionally, TLTF was detected in CSF from late stage patients by Western blotting with the anti-TLTF specific monoclonal antibody MO3. Conclusions/Significance: These findings suggest a new possibility for disease diagnosis with focus on involvement of the CNS through detection of TLTF and anti-TLTF antibodies in the CSF. © 2013 Abdulla et al.
Authors & Co-Authors
Abdulla, Maha Hamadien
Saudi Arabia, Riyadh
King Khalid University Hospital
United States, La Jolla
Center for Discovery and Innovation in Parasitic Diseases
Bakhiet, Raga M.
Bahrain, Manama
Arabian Gulf University
Lejon, Veerle
Belgium, Antwerpen
Universiteit Antwerpen
Andersson, Jan P.
Sweden, Stockholm
Karolinska Universitetssjukhuset
McKerrow, James Hobson
United States, La Jolla
Center for Discovery and Innovation in Parasitic Diseases
Al-Obeed, Omar Abdullah
Saudi Arabia, Riyadh
King Khalid University Hospital
Harris, Robert Adam
Sweden, Stockholm
Karolinska Universitetssjukhuset
Statistics
Citations: 7
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0079281
e-ISSN:
19326203