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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection
PLoS Pathogens, Volume 6, No. 9, Article e01108, Year 2010
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Description
Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci. © 2010 Hiller et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s001.ppt
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s002.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s003.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s004.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s005.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s006.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s007.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC2940740/bin/ppat.1001108.s008.doc
Authors & Co-Authors
Hiller, N. Luisa
United States, Pittsburgh
Allegheny General Hospital
Ahmed, Azad
United States, Pittsburgh
Allegheny General Hospital
Powell, Evan
United States, Pittsburgh
Allegheny General Hospital
Martin, Darren Patrick
South Africa, Cape Town
University of Cape Town
Eutsey, Rory
United States, Pittsburgh
Allegheny General Hospital
Earl, Josh
United States, Pittsburgh
Allegheny General Hospital
Janto, Benjamin
United States, Pittsburgh
Allegheny General Hospital
Boissy, Robert J.
United States, Omaha
University of Nebraska Medical Center
Hogg, Justin
United States, Pittsburgh
Allegheny General Hospital
Barbadora, Karen
United States, Pittsburgh
Upmc Children’s Hospital of Pittsburgh
Sampath, Rangarajan
United States, Des Plaines
Abbott Molecular Inc.
Lonergan, Shaun
United States, Des Plaines
Abbott Molecular Inc.
Post, J. Christopher
United States, Pittsburgh
Allegheny General Hospital
United States, Philadelphia
Drexel University College of Medicine
Hu, Fen Z.
United States, Pittsburgh
Allegheny General Hospital
United States, Philadelphia
Drexel University College of Medicine
Ehrlich, Garth D.
United States, Pittsburgh
Allegheny General Hospital
United States, Philadelphia
Drexel University College of Medicine
Statistics
Citations: 141
Authors: 15
Affiliations: 6
Identifiers
Doi:
10.1371/journal.ppat.1001108
ISSN:
15537366
e-ISSN:
15537374
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health