Isolation and Identification of Brevibacillus and Escherichia spp. from Urinary Tract of Infected Patients

Urinary tract infection (UTI) is a major public health concern, especially in women, across the globe. Among the uropathogens, E. coli is most implicated in UTI infected patients. Urine samples were collected, cultured and subcultured using different media to determine the morphology and biochemical properties of the bacteria in the samples. DNA samples from the pure cultures were amplified, sequenced and subjected to BLAST analysis. Isolates identified in the urine samples belonged to the genus Escherichia, Klebsiella, Proteus, Enterobacter, Staphylococci and Brevibacilli. The presence of Escherichia marmotae and Brevibacillus panacihumi was confirmed from the result of the BLAST. The study implicated E. marmotae and B. panacihumi in UTI infection in the studied population. Further study is encouraged to analyze the virulence genes responsible for their pathogenicity.