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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Genetic diversity of food-isolated Salmonella strains through Pulsed Field Gel Electrophoresis (PFGE) and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
PLoS ONE, Volume 8, No. 12, Article e81315, Year 2013
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Description
All over the world, the incidence of Salmonella spp contamination on different food sources like broilers, clams and cow milk has increased rapidly in recent years. The multifaceted properties of Salomnella serovars allow the microorganism to grow and multiply in various food matrices, even under adverse conditions. Therefore, methods are needed to detect and trace this pathogen along the entire food supply network. In the present work, PFGE and ERIC-PCR were used to subtype 45 Salmonella isolates belonging to different serovars and derived from different food origins. Among these isolates, S. Enteritidis and S. Kentucky were found to be the most predominant serovars. The Discrimination Index obtained by ERICPCR (0.85) was slightly below the acceptable confidence value. The best discriminatory ability was observed when PFGE typing method was used alone (DI = 0.94) or combined with ERIC-PCR (DI = 0.93). A wide variety of profiles was observed between the different serovars using PFGE or/and ERIC-PCR. This diversity is particularly important when the sample origins are varied and even within the same sampling origin. © 2013 Fendri, et al.
Authors & Co-Authors
Fendri, Imen
Tunisia, Sfax
Faculté Des Sciences de Sfax
France, Rennes
L'institut Agro Rennes-angers
Ben Hassena, Amal
Tunisia, Sfax
Faculté Des Sciences de Sfax
Grosset, Noël
France, Rennes
L'institut Agro Rennes-angers
Barkallah, Mohamed
Tunisia, Sfax
Faculté Des Sciences de Sfax
Khannous, Lamia
Tunisia, Sfax
Faculté Des Sciences de Sfax
Chuat, Victoria
France, Rennes
Science et Technologie du Lait et de L'oeuf Stlo
Gautier, Michel
France, Rennes
L'institut Agro Rennes-angers
Gdoura, Radhouane
Tunisia, Sfax
Faculté Des Sciences de Sfax
Statistics
Citations: 51
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0081315
e-ISSN:
19326203
Research Areas
Food Security
Genetics And Genomics
Infectious Diseases
Study Design
Cohort Study