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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
The genome of mycobacterium Africanum West African 2 reveals a lineage-specific locus and genome erosion common to the M. tuberculosis complex
PLoS Neglected Tropical Diseases, Volume 6, No. 2, Article e1552, Year 2012
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Description
Background: M. africanum West African 2 constitutes an ancient lineage of the M. tuberculosis complex that commonly causes human tuberculosis in West Africa and has an attenuated phenotype relative to M. tuberculosis. Methodology/Principal Findings: In search of candidate genes underlying these differences, the genome of M. africanum West African 2 was sequenced using classical capillary sequencing techniques. Our findings reveal a unique sequence, RD900, that was independently lost during the evolution of two important lineages within the complex: the "modern" M. tuberculosis group and the lineage leading to M. bovis. Closely related to M. bovis and other animal strains within the M. tuberculosis complex, M. africanum West African 2 shares an abundance of pseudogenes with M. bovis but also with M. africanum West African clade 1. Comparison with other strains of the M. tuberculosis complex revealed pseudogenes events in all the known lineages pointing toward ongoing genome erosion likely due to increased genetic drift and relaxed selection linked to serial transmission-bottlenecks and an intracellular lifestyle. Conclusions/Significance: The genomic differences identified between M. africanum West African 2 and the other strains of the Mycobacterium tuberculosis complex may explain its attenuated phenotype, and pave the way for targeted experiments to elucidate the phenotypic characteristic of M. africanum. Moreover, availability of the whole genome data allows for verification of conservation of targets used for the next generation of diagnostics and vaccines, in order to ensure similar efficacy in West Africa. © 2012 Bentley et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3289620/bin/pntd.0001552.s001.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3289620/bin/pntd.0001552.s002.xlsx
Authors & Co-Authors
Bentley, Stephen D.
United Kingdom, Hinxton
Wellcome Sanger Institute
Comas, Iñaki T.
Spain, Valencia
Centre for Public Health Research
United Kingdom, London
Mrc National Institute for Medical Research
Bryant, Josephine
United Kingdom, Hinxton
Wellcome Sanger Institute
Walker, Danielle Carey
United Kingdom, Hinxton
Wellcome Sanger Institute
Smith, Noel H.
United Kingdom, Addlestone
Animal and Plant Health Agency
United Kingdom, Brighton
University of Sussex
Harris, Simon R.
United Kingdom, Hinxton
Wellcome Sanger Institute
Thurston, Scott A.J.
United Kingdom, Hinxton
Wellcome Sanger Institute
Gagneux, Sébastien P.
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
Universitat Basel
Wood, Jonathan M.D.
United Kingdom, Hinxton
Wellcome Sanger Institute
Antonio, Martin
Gambia, Banjul
Medical Research Council Laboratories Gambia
Quail, Michael A.
United Kingdom, Hinxton
Wellcome Sanger Institute
Gehre, Florian
Gambia, Banjul
Medical Research Council Laboratories Gambia
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Adegbola, Richard A.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Parkhill, Julian
United Kingdom, Hinxton
Wellcome Sanger Institute
De-Jong, Bouke Catherine
Gambia, Banjul
Medical Research Council Laboratories Gambia
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
United States, New York
New York University
Statistics
Citations: 15
Authors: 15
Affiliations: 10
Identifiers
Doi:
10.1371/journal.pntd.0001552
ISSN:
19352727
e-ISSN:
19352735
Research Areas
Genetics And Genomics
Study Locations
Multi-countries