Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Rare PfCSP C-terminal antibodies induced by live sporozoite vaccination are ineffective against malaria infection
Journal of Experimental Medicine, Volume 215, No. 1, Year 2018
Notification
URL copied to clipboard!
Description
Antibodies against the central repeat of the Plasmodium falciparum (Pf) circumsporozoite protein (CSP) inhibit parasite activity and correlate with protection from malaria. However, the humoral response to the PfCSP C terminus (C-PfCSP) is less well characterized. Here, we describe B cell responses to C-PfCSP from European donors who underwent immunization with live Pf sporozoites (PfSPZ Challenge) under chloroquine prophylaxis (PfSPZ-CVac), and were protected against controlled human malaria infection. Out of 215 PfCSP-reactive monoclonal antibodies, only two unique antibodies were specific for C-PfCSP, highlighting the rare occurrence of C-PfCSP-reactive B cells in PfSPZ-CVac-induced protective immunity. These two antibodies showed poor sporozoite binding and weak inhibition of parasite traversal and development, and did not protect mice from infection with PfCSP transgenic Plasmodium berghei sporozoites. Structural analyses demonstrated that one antibody interacts with a polymorphic region overlapping two T cell epitopes, suggesting that variability in C-PfCSP may benefit parasite escape from humoral and cellular immunity. Our data identify important features underlying C-PfCSP shortcomings as a vaccine target. © 2018 Scally et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC5748854/bin/JEM_20170869_sm.pdf
Authors & Co-Authors
Scally, Stephen W.
Canada, Toronto
Sickkids Research Institute
Murugan, Rajagopal
Germany, Heidelberg
German Cancer Research Center
Bosch, Alexandre
Canada, Toronto
Sickkids Research Institute
Triller, Gianna
Germany, Heidelberg
German Cancer Research Center
Costa, Giulia
Germany, Berlin
Max Planck Institute for Infection Biology
Mordmüller, Benjamin G.
Germany, Tubingen
Eberhard Karls Universität Tübingen
Kremsner, Peter G.
Germany, Tubingen
Eberhard Karls Universität Tübingen
Sim, Betty Kim Lee
United States, Rockville
Sanaria Inc.
Hoffman, Stephen L.
United States, Rockville
Sanaria Inc.
Levashina, Elena A.
Germany, Berlin
Max Planck Institute for Infection Biology
Wardemann, Hedda
Germany, Heidelberg
German Cancer Research Center
Julien, Jean Philippe
Canada, Toronto
Sickkids Research Institute
Canada, Toronto
University of Toronto
Statistics
Citations: 60
Authors: 12
Affiliations: 6
Identifiers
Doi:
10.1084/jem.20170869
ISSN:
00221007
Research Areas
Infectious Diseases
Maternal And Child Health