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Polysaccharide from Pimpinella anisum seeds: Structural characterization, anti-inflammatory and laser burn wound healing in mice

International Journal of Biological Macromolecules, Volume 156, Year 2020

The polysaccharide preparation from Pimpinella anisum seeds (PAP) was isolated and characterized to evaluate its laser burn wound-healing and anti-inflammatory activities in mice. The structure characterization of PAP by Infra-red spectrometry (IR), Nuclear magnetic resonance (NMR), Gas chromatogram-Mass spectrometer (GC–MS) and colorimetric methods revealed an optimum yield of 8.84%, a high quantity of carbohydrate (64.75%) and low levels of lipids, protein and sulfate. Galactose (33.47%), β-D-Glucose (26.71%) and α-D-Mannose (18.21%) were the major monosaccharides components presenting in PAP, and a smaller amounts of β-D-Galactose, D-Fructose, α-D-Glucose, α-L-Galactose and arabinose were detected. PAP showed noticeable antioxidant and antibacterial properties. The anti-inflammatory activity of PAP in the carrageenan-induced paw edema model in mice, demonstrated by reduced edema and cellular infiltration, and oxidative stress markers in muscle tissue. A beneficial wound healing effect was also revealed. The topical application of PAP based gel on laser burn lesions accelerates wound contraction, the re-epithelization and remodeling phases after seven days of treatment. The results demonstrated that PAP is a novel promising source of natural wound healing and anti-inflammatory drugs. The high content and varied PAP monosaccharides seem to be responsible for the observed biological activities.
Statistics
Citations: 44
Authors: 8
Affiliations: 4
Research Areas
Cancer