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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Comparative study of single and nested PCR for the detection of proviral HIV2 DNA
Research in Virology, Volume 145, No. C, Year 1994
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Description
We have tested seven pairs of primers for the detection of HIV2 DNA by single PCR in positive cultures from 21 infected patients. Four of these primer pairs were then used in a comparative study of single and nested PCR for the detection of HIV2 in fresh lymphocytes from 33 patients infected by the virus. HIV2 DNA was detected in 17 of the 33 patients (51.5%) by single PCR and 29/33 (88%) by nested PCR. All the patients negative in both nested and single PCR were asymptomatic and had CD4+ lymphocyte counts of at least 500 × 106/l. This lack of PCR sensitivity for the detection of proviral HIV2 DNA in fresh lymphocytes cannot be totally attributed to genetic variability and may be related to a low viral load in asymptomatic HIV2-infected patients. © 1994 Institut Pasteur/Elsevier.
Authors & Co-Authors
Loussert-Ajaka, Ibtissam
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Simon, Franćois
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Farfara, Isabelle
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Houhou-Fidouh, Nadhira
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Couto-Fernandez, José Carlos
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Dazza, Marie Christine
France, Paris
Inserm
Brun-Vézinet, Françoise
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Statistics
Citations: 7
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/S0923-2516(07)80039-2
ISSN:
09232516
Research Areas
Genetics And Genomics