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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Melatonin prevents the free radical and MADD metabolic profiles induced by antituberculosis drugs in an animal model
Journal of Pineal Research, Volume 38, No. 2, Year 2005
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Description
The objective was to determine the effect of combined antituberculosis (anti-TB) drug therapy and an antioxidant, melatonin, on the free radical and organic acid profiles in an experimental rat model. A combined anti-TB drug, Rifater, consisting of 12.0 mg rifampicin, 0.8 mg isoniazid, and 23.0 mg pyrazinamide and 18.56 μg melatonin/kg body weight per day (corresponding to average physiological human intake) were orally administered to Sprague-Dawley rats. Hydroxyl radical production was monitored by quantifying 2,3-dihydroxybenzoic acid produced after intraperitonial sodium salicylate injections. Organic acid extractions and gas chromatography-coupled mass spectrometry analyses were performed on collected urine samples. The results show hydroxyl radicals (P = 0.0019) and organic acids (P-value range: 0.037 to < 0.001), characteristic of a multiple acyl-CoA dehydrogenase defect (MADD), were elevated with Rifater treatment and these elevations were significantly lowered with melatonin pretreatment (P-value range: 0.031 to < 0.001), probably because of its inherent antioxidant activity. We conclude that hydroxyl radical production and an increased organic acid profile induced by anti-TB medication indicates inhibition of the electron transport chain. We also conclude that free radicals leading to clinical symptoms associated with an MADD metabolic profile induced by anti-TB treatment could be alleviated by melatonin intervention. Copyright © Blackwell Munksgaard, 2004.
Authors & Co-Authors
Loots, Du Toit
South Africa, Potchefstroom
North-west University
Wiid, Ian J.F.
South Africa, Potchefstroom
Mrc Ctr. for Molec. and Cell. Biol.
South Africa, Stellenbosch
Stellenbosch University
Page, Benedict John
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Mienie, L. J.
South Africa, Potchefstroom
North-west University
Van Helden, Paul D.
South Africa, Potchefstroom
Mrc Ctr. for Molec. and Cell. Biol.
Statistics
Citations: 24
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/j.1600-079X.2004.00176.x
ISSN:
07423098
Research Areas
Infectious Diseases
Noncommunicable Diseases
Study Design
Randomised Control Trial