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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Genomic characterization of enteroaggregative Escherichia coli from children in Mali
Journal of Infectious Diseases, Volume 205, No. 3, Year 2012
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Description
Background. Enteroaggregative Escherichia coli (EAEC) is a cause of epidemic and sporadic diarrhea, yet its role as an enteric pathogen is not fully understood. Methods. We characterized 121 EAEC strains isolated in 2008 as part of a case-control study of moderate to severe acute diarrhea among children 0-59 months of age in Bamako, Mali. We applied multiplex polymerase chain reaction and comparative genome hybridization to identify potential virulence factors among the EAEC strains, coupled with classification and regression tree modeling to reveal combinations of factors most strongly associated with illness. Results. The gene encoding the autotransporter protease SepA, originally described in Shigella species, was most strongly associated with diarrhea among the EAEC strains tested (odds ratio, 5.6 [95% confidence interval, 1.92-16.17]; P =. 0006). In addition, we identified 3 gene combinations correlated with diarrhea: (1) a clonal group positive for sepA and a putative hemolysin; (2) a group harboring the EAST-1 enterotoxin and the flagellar type H33 but no other previously identified EAEC virulence factor; and (3) a group carrying several of the typical EAEC virulence genes. Conclusion. Our data suggest that only a subset of EAEC strains are pathogenic in Mali and suggest that sepA may serve as a valuable marker for the most virulent isolates. © The Author 2011. Published by Oxford University Press on behalf of the Infectious.
Authors & Co-Authors
Boisen, Nadia
Denmark, Copenhagen
Statens Serum Institut
United States, Charlottesville
University of Virginia School of Medicine
Scheutz, Flemming F.
Denmark, Copenhagen
Statens Serum Institut
Rasko, David A.
Unknown Affiliation
Redman, Julia
Unknown Affiliation
Persson, Søren
United States, Baltimore
Institute for Genome Sciences
Simon, Jakub K.
United States, Baltimore
University of Maryland School of Medicine
Kotloff, Karen L.
United States, Baltimore
University of Maryland School of Medicine
Levine, Myron M.
United States, Baltimore
University of Maryland School of Medicine
Sow, Samba Ousmane
Mali, Bamako
Center for Vaccine Development
Tamboura, Boubou
Mali, Bamako
Center for Vaccine Development
Touré, Aliou Y.
Mali, Bamako
Center for Vaccine Development
Malle, Dramane
Mali, Bamako
Center for Vaccine Development
Panchalingam, Sandra
United States, Baltimore
University of Maryland School of Medicine
Krogfelt, Karen Angeliki
Denmark, Copenhagen
Statens Serum Institut
Nataro, James Paul
United States, Charlottesville
University of Virginia School of Medicine
Statistics
Citations: 167
Authors: 15
Affiliations: 5
Identifiers
Doi:
10.1093/infdis/jir757
ISSN:
00221899
Research Areas
Genetics And Genomics
Maternal And Child Health
Study Design
Case-Control Study
Study Locations
Mali