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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Fasciola gigantica excretory-secretory products for immunodiagnosis and prevention of sheep fasciolosis
Veterinary Parasitology, Volume 149, No. 3-4, Year 2007
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Description
Excretory-secretory products (ESP) products of ex vivo Fasciola gigantica adult worms were used for immunodiagnosis of sheep experimental infection with F. gigantica and natural infection with Fasciola spp. by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Specific IgG antibody binding to native or denatured ESP was detected as early as 2 weeks after experimental sheep infection with 100 or 200 metacercariae. No specific IgG antibody binding was displayed by sera obtained from 192 sheep considered to be Fasciola- and other parasite-free by microscopic examination of bile and feces. Additionally, sera from 200 apparently Fasciola-free sheep, yet infected with other parasites, were all negative. The data, thus, indicated that ESP-based ELISA reached nearly 100% sensitivity and specificity in immunodiagnosis of sheep fasciolosis. As expected, the ESP molecules were immunogenic in sheep eliciting interleukin-12p40 mRNA response and considerable amounts of antibodies, which were able to bind to the surface of newly excysted juvenile worms as judged by membrane indirect immunofluorescence, and mediate their attrition via antibody-dependent cell-mediated cytotoxicity. The ESP-induced cellular and humoral immune responses were associated with a modest reduction in worm count, yet with a highly significant (P < 0.0001) decrease in size of recovered worms, thus suggesting that ESP immunization might be a safe and cost-effective strategy for reducing transmission of the infection. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
El Ridi, R.
Egypt, Giza
Faculty of Science
Salah, Mohamed
Egypt, Giza
Theodor Bilharz Research Institute
Wagih, A.
Egypt, Giza
Faculty of Science
William, H.
Egypt, Giza
Faculty of Science
Tallima, Hatem A.M.
Egypt, Giza
Faculty of Science
El-Shafie, M. H.
Egypt, Cairo
Egyptian Ministry of Agriculture, Cairo
Abdel-Khalek, Tarek Mohammad
Egypt, Cairo
Egyptian Ministry of Agriculture, Cairo
El Amir, A. M.
Egypt, Giza
Faculty of Science
Abo Ammou, F. F.
Egypt, Cairo
Egyptian Ministry of Agriculture, Cairo
Motawi, H.
Egypt, Cairo
Egyptian Ministry of Agriculture, Cairo
Statistics
Citations: 38
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1016/j.vetpar.2007.08.024
ISSN:
03044017
Research Areas
Maternal And Child Health