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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Distribution of the molecular forms of Anopheles gambiae and pyrethroid knock down resistance gene in Nigeria
Acta Tropica, Volume 95, No. 3, Year 2005
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Description
We investigated the distribution of the molecular M and S forms of Anopheles gambiae and the knock down resistance (kdr) gene associated with pyrethroid and DDT resistance in A. gambiae s.s. at 13 localities across Nigeria. Two-three days old adult female mosquito reared from larval collections were tested using standard WHO procedures, diagnostic test kits and impregnated papers to assess their pyrethroid resistance status. Specimens were identified by PCR assays and characterized for the kdr gene. DNA from adult A. gambiae s.s. collected from human dwellings were also tested for the presence of the kdr gene. The overall collection was a mix of the molecular M and S forms across the mangrove (63:37%), forest (56:44%), and transitional (36:64%) ecotypes, but almost a pure collection of the S form in the Guinea and Sudan-savanna. Results of insecticide susceptibility tests showed that mosquitoes sampled at seven localities were susceptible to permethrin, deltamethrin, and DDT, but populations of A. gambiae resistant to these insecticides were recorded at six other localities mainly in the transitional and Guinea-savanna ecotypes. The kdr gene was found only in the molecular S forms, including areas where both forms were sympatric. The overall kdr frequency was low: <47% in forest, 37-48% in the transitional, and 45-53% in Guinea-savanna. The data suggest that pyrethroid resistance in A. gambiae in Nigeria is not as widespread when compared to neighbouring West African countries. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Awolola, Taiwo Samson
Nigeria, Yaba
Nigerian Institute of Medical Research
Oyewole, Isaac O.
Nigeria, Ilishan-remo
Babcock University
Amajoh, Chioma N.
Nigeria, Abuja
Federal Ministry of Health
Idowu, Emmanuel Taiwo
Nigeria, Yaba
Nigerian Institute of Medical Research
Ajayi, Morakinyo B.
Nigeria, Yaba
Nigerian Institute of Medical Research
Oduola, Adedayo Olatunbosun
Nigeria, Ibadan
University of Ibadan
Manafa, O. U.
Nigeria, Yaba
Nigerian Institute of Medical Research
Ibrahim, K.
Nigeria, Ibadan
University of Ibadan
Koekemoer, Lizette Leonie
South Africa, Johannesburg
University of the Witwatersrand
South Africa, Johannesburg
National Institute for Communicable Diseases
Coetzee, Maureen
South Africa, Johannesburg
University of the Witwatersrand
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 93
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1016/j.actatropica.2005.06.002
ISSN:
0001706X
Research Areas
Genetics And Genomics
Study Locations
Guinea
Nigeria
Sudan
Participants Gender
Female