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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Proteomic analysis of seminal fluid from men exhibiting oxidative stress
Reproductive Biology and Endocrinology, Volume 11, No. 1, Article 85, Year 2013
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Description
Background: Seminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and sperm dysfunction in infertile men.Methods: This prospective study consisted of 52 subjects: 32 infertile men and 20 healthy donors. Once semen and oxidative stress parameters were assessed (ROS, antioxidant concentration and DNA damage), the subjects were categorized into ROS positive (ROS+) or ROS negative (ROS-). Seminal plasma from each group was pooled and subjected to proteomics analysis. In-solution digestion and protein identification with liquid chromatography tandem mass spectrometry (LC-MS/MS), followed by bioinformatics analyses was used to identify and characterize potential biomarker proteins.Results: A total of 14 proteins were identified in this analysis with 7 of these common and unique proteins were identified in both the ROS+ and ROS- groups through MASCOT and SEQUEST analyses, respectively. Prolactin-induced protein was found to be more abundantly present in men with increased levels of ROS. Gene ontology annotations showed extracellular distribution of proteins with a major role in antioxidative activity and regulatory processes.Conclusions: We have identified proteins that help protect against oxidative stress and are uniquely present in the seminal plasma of the ROS- men. Men exhibiting high levels of ROS in their seminal ejaculate are likely to exhibit proteins that are either downregulated or oxidatively modified, and these could potentially contribute to male infertility. © 2013 Sharma et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Sharma, Rakesh Kumar
United States, Cleveland
Cleveland Clinic Foundation
Agarwal, Ashok K.
United States, Cleveland
Cleveland Clinic Foundation
Mohanty, Gayatri
United States, Cleveland
Cleveland Clinic Foundation
India, Cuttack
Ravenshaw University
Du Plessis, Stefan Stephanus
South Africa, Stellenbosch
Stellenbosch University
Gopalan, Banu
United States, Cleveland
Cleveland Clinic Foundation
Willard, Belinda
United States, Cleveland
Cleveland Clinic Foundation
Yadav, Satya P.
United States, Cleveland
Cleveland Clinic Foundation
Sabanegh, Edmund
United States, Cleveland
Cleveland Clinic Foundation
Statistics
Citations: 91
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1186/1477-7827-11-85
e-ISSN:
14777827
Research Areas
Genetics And Genomics
Sexual And Reproductive Health
Study Design
Cohort Study
Participants Gender
Male